Data readout from Olink panels
Please note that the data analysis steps below will be taken care of for you if you decide to send your samples to our dedicated, highly skilled Analysis Service team, who will provide you with a comprehensive NPX data/quality control report after your study is completed.
If more extensive data analysis services are required, our team of Olink biostatisticians can help you with customized statistical analysis – see our statistical packages.
If you intend to run our panels in your own lab, or simply want to know more details, please read on below!
Detection and sample analysis is performed by high-throughput real-time PCR analysis using the Fluidigm® BioMark™ HD System. This PCR platform is a truly high capacity system which enables simultaneous detection of 96 analytes in 96 samples creating 9,216 datapoints from a single run. The BioMark HD platform uses microfluidic distribution of sample and assays requiring only 7 nL reactions and takes less than 3 hours to complete. The BioMark HD platform consists of a real-time PCR instrument, 96×96 Dynamic Array™ IFC for multiplexing the samples and biomarkers, and an IFC loader to load the 96×96 Dynamic Array IFC. More information can be found on the Fluidigm website.
(Fluidigm, BioMark, and Dynamic Array are trademarks or registered trademarks of Fluidigm Corporation).
Data generation and quality control with Olink® NPX Manager
For easier data analysis Olink Proteomics has developed a software, Olink NPX Manager, which can be used for data generated with the Olink panels and the Fluidigm BioMark real-time PCR instrument. This is an easy-to-use tool that lets you import, quality-control and normalize your Olink data, providing you with Normalized Protein eXpression (NPX) values for further data analysis.
If you want to purchase an Olink NPX Manager license, please contact your Business Development Manager at Olink Proteomics, or email firstname.lastname@example.org.
To see more details about this software, you can download the Olink NPX Manager User Guide here.
Setup in the Fluidigm Biomark software
To obtain the biomarker list, import the panel-specific .plt file directly into the Fluidigm RT PCR software. Make sure to load the Primer Plate according to figure 3 in the User Manual. Please note that the detection setup.plt and setup.plt files should be saved to your computer first before imported into the Fluidigm RT PCR software. The .plt files can be downloaded below (right-click and “Save link as..” to your computer or device).
Default Sample Setup.plt
Cardiometabolic Detector Setup.plt
Cell Regulation Detector Setup.plt
Cardiovascular II Detector Setup.plt
Cardiovascular III Detector Setup.plt (note: this is for the latest version of the Cardiovascular III panel, article number 94610, released July 2017).
Cardiovascular III Detector Setup.plt
Development Detector Setup.plt
Immune Response Detector Setup.plt
Immuno-Onc Detector Setup.plt
Inflammation Detector Setup.plt
Metabolism Detector Setup.plt
Neurology Detector Setup.plt
Oncology II Detector Setup.plt
Organ Damage Detector Setup.plt
Mouse Exploratory Detector Setup.plt