• Proximity Hybridizing Chain Reaction

    “The gamechanger in molecular technologies”

    Example: ProxHCR staining of amylase on pancreas cancer tissue.

    Introduction

    Our proprietary ProxHCR method combines proximal binding by pairs of antibodies, enabling dual-recognition of desired target, with cost-efficient and highly sensitive signal amplification via a hybridization chain reaction (HCR).

    The principle

    ProxHCR is a proximity-dependent technique developed as an improved compliment to regular IF and IHC, and to tackle current limitations of protein detection in situ. ProxHCR utilizes two antibodies to bind close together to give rise to detectable signals, thus improving specificity of detection. Dual recognition enables protein-protein-interactions (PPIs) and protein-modifications (PTMs) by applying antibodies directed against different proteins or towards a protein and general phosphorylation site. When two such affinity probes bind in proximity they can be activated to reveal an initiator sequence through our proprietary mechanism. The initiator sequence is then used as a template for localized hybridization chain reaction (HCR) signal amplification. Analysis is performed under a microscope much like regular IHC and IF but with much higher specificity. As the dual recognition demand greatly increases detection specificity, completely reduces the risk of false positives and enables study of protein interaction or protein modification, the signal amplification provides one of the market highest sensitivity. ProxHCR generates an interpretable and uniform signal readout without losing special information.

     

    Assay specific kits under development