PEA Technology

Proseek principle

The Proseek reagents are based on PEA, which is a Proximity Extension Assay technology. A pair of oligonucleotide labeled antibodies, Proseek probes, are allowed to pair-wise bind to the target protein present in the sample in a homogeneous assay, without washing steps. When the two Proseek probes are in close proximity, a new PCR target sequence is formed by a proximity dependent DNA polymerization event. The resulting sequence is subsequently detected and quantified using standard real-time PCR.