Duolink products

The Duolink^® products enable you to detect and individually count single endogenous proteins, protein-protein interactions and protein phosphorylations in fixed cells and tissues samples. Use Duolink dual recognition to study cell signaling pathways and receptor activation events or single recognition to quantify the level of protein expression.

The Duolink kit contains oligonucleotide labeled secondary antibodies, so called PLA probes, which are used together with your own target specific primary antibodies, monoclonal or polyclonal. The PLA probes will generate a fluorescent or luminescent signal, detectable in a fluorescence or brightfield microscope, only when the two primary antibodies have bound in close proximity.

A complete Duolink kit comprises one of each:

• PLA probe PLUS

• PLA probe MINUS

• Detection Reagents

The complete kit contains all buffers and enzymes needed for performing an in situ PLA, except for primary antibodies. You may choose from reagent kit sizes of 30 or 100 assays of 40µl each. The choice of PLA probes, depends on your primary antibodies and the application you have chosen.

Duolink^® Probemaker is a product enabling quick and convenient conjugation of the PLA oligo arms directly to primary antibodies. Directly conjugating primary antibodies enables same species monoclonal or polyclonal use in a Duolink assay. A consequence of this feature is the possibility to study homodimeric protein complexes – an ability unique to Duolink.
Simply include Probemaker probes instead of PLA probes in a Duolink kit.

Accessories such as Control Kit, Mounting Media and Wash Buffers are also available.

Contract service

To enable clients in academia and industry to rapidly take maximum advantage of our technology platforms, Olink Bioscience offers assay development and reagent manufacturing services for customized applications on a contract service basis. For further information contact Peter Karlberg at info@olink.com

For contract services we also collaborate with MicroMorph AB who is a qualified provider of Duolink contract research services.

  Scheelevägen 22, 223 63 Lund, Phone +46 (0) 46 19 11 15, Fax +46 (0) 46 39 75 00, info@micromorph.se