Proseek Multiplex: A Precision proteomics solution for targeted protein biomarker discovery
Proseek® Multiplex enables faster, better-informed decisions in human protein biomarker research by providing high-multiplex immunoassays that do not compromise on either data quality or performance. This helps scientists to bring new insights into disease processes, improve disease detection, and contribute to a better understanding of biology. Identifying multiple proteins that form a signature is more powerful and reliable than looking at a single protein. Achieving this requires casting a wide net, with studies that examine many different proteins simultaneously, in large numbers of human samples. Olink enables this today, providing an ever-expanding range of panels for high quality precision biomarker discovery.
………precision proteomics – for life!
Proseek Multiplex gives you:
- High quality data you can trust – exceptional readout specificity from our Proximity Extension Assay (PEA) technology – effective QC tools built in to our assays (read our FAQ)
- The high throughput your research deserves – 92 human protein biomarkers measured simultaneously with 96 samples in one panel – results in 24 hours
- The most effective use of your precious samples – only 1 µL sample consumed per panel – a wide range of biological sample types addressed
- The flexibility to best meet your needs – use our Proseek Multiplex kits in your own lab, or let our experts run the assays for you with the Proseek Multiplex Analysis Service
Our innovative technology
Proseek Multiplex is able to achieve such a high level of multiplexing while maintaining exceptional data quality thanks to our proprietary Proximity Extension Assay (PEA) technology. Each biomarker is addressed by a matched pair of antibodies, coupled to unique, partially complementary oligonucleotides, and measured by quantitative real-time PCR. This dual recognition, DNA-coupled method provides exceptional readout specificity, since unlike in many other immunoassay formats, any antibody cross-reactivity that may occur during the multiplexed assay is excluded from the detection process. Go to the PEA technology page to read more about how it works, and see the movie!
PEA technology enables high multiplexing levels without compromising on data quality: the figure compares dCq-values from single assays for Growth Hormone (GH) and Matrix Metalloproteinase (MMP-7) with the equivalent assays performed in a full 96-plex reaction.
Proseek Multiplex can provide accurate quantification below picogram per milliliter levels even in small samples from, for example, needle biopsies or micro dialysis. This minimal sample consumption makes Proseek Multiplex especially suitable for retroactively mining precious biobanked serum samples for new multivariate biomarker signatures. The opportunities are enormous for powerful analysis of multivariate biomarker patterns. Proseek Multiplex reactions are quantified by real-time PCR using the Fluidigm BioMark™ HD real-time PCR platform. The assay generates as much as 9,216 datapoints per run which can then be analyzed with GeneEx software, equipped with the user-friendly Proseek Multiplex data import wizard.
Each panel is focused on a specific area of disease or key biology process, targeting 92 established and/or exploratory biomarkers. All assays are rigorously quality controlled and our validation data is made freely available. Currently there are six disease area-focus panels available: two for cardiovascular disease (CVD) and one each for neurology, oncology, inflammation and immuno-oncology. These are complemented by three exploratory panels, focused on immune response, metabolism and organ damage.
Together, our panels span a broad dynamic range of protein concentrations. This is optimized within each panel for overall assay performance, and designed to cover expected ranges in normal and pathological samples.
Example dynamic ranges of selected panels
Our biomarker assay panels are available with the flexibility to best suit your needs. You can choose whether to purchase the panels as kits and run them in your own lab, or send your sample to us and let our experts here at Olink do this for you via our fee-for-service offering, Proseek Multiplex Analysis Service.