• Proseek Multiplex Immuno-Onc I

    A targeted protein biomarker discovery solution for immuno-oncology

    Immunotherapy is one of the most exciting and rapidly developing approaches to cancer therapy. Strategies that aim to strengthen the patients’ own immune system to fight tumor cells and to break tolerance in the tumor microenvironment are beginning to show great promise, with several successful clinical trials reported and some successes dealing with several types of tumors in patients with poor prognosis. I-O icon

    Proseek® Multiplex Immuno-Onc I96×96 is a high-throughput, multiplex immunoassay enabling analysis of 92  protein biomarkers across 96 samples simultaneously. This high level of multiplexing is achieved without any compromise on data quality, thanks to our proprietary Proximity Extension Assay (PEA) technology

    Non-invasive biomarkers for the immuno-oncology area offer great potential benefits in helping to better understand the underlying pathophysiology, study therapeutic efficacy and to stratify patients for clinical trials. This panel provide the immuno-oncology field with a tool that casts a relatively broad net (simultaneous analysis of 92 proteins), while enabling a targeted approach via the careful selection of relevant assays available. The biomarkers selected in consultation with experts in the field include proteins involved in processes such as promotion and inhibition of tumor immunity, chemotaxis, vascular & tissue remodeling, apoptosis & cell killing and metabolism & autophagy.

    An overview of how the 92 biomarker assays in the panel are classified according to Biological Process, Disease Area, Tissue Expression and Protein Class can be seen in the charts below. These charts are interactive: click on a chart-bar or pie-segment to see the list of biomarkers in that classification. Each item in the list links to the detailed biomarker page with validation data.

    Biological Process

    Disease Area

    Tissue Expression

    Protein Class

                                                         

    A comprehensive list of all protein biomarkers that can be measured using the currently available Proseek Multiplex panels can be seen on the biomarker library page.

    Note: A printable list of the biomarker assays available in this panel, as well as a full data validation document, can be downloaded from our Document download center >>

    If you prefer not to run the assay yourself, let our experts here at Olink do this for you via our Proseek Multiplex Analysis Service.

    The biomarker pages linked to below include calibrator curves that show the performance of each assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens, with data presented as Normalized Protein eXpression (NPX) values plotted against protein concentration (in pg/mL). Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please view the FAQ.

  • Search all our biomarkers

      Biomarkers in Proseek Multiplex Immuno-Onc I

      • Adenosine Deaminase (ADA)

        ADA is a key enzyme of the purine metabolism and it is involved in the breakdown of adenosine into nucleic acids. In humans it is primarily involved in the development and maint...

      • Adhesion G-protein coupled receptor G1 (ADGRG1)

        Receptor involved in cell adhesion and probably in cell-cell interactions. Mediates cell matrix adhesion in developing neurons and hematopoietic stem cells. Receptor for collage...

      • Angiopoietin-1 (ANG-1)

        ANG-1 is a member of the vascular growth factor family, and via its interactions with the TEK/TIE2 receptor, plays an important role in the regulation of angiogenesis.During emb...

      • Angiopoietin-1 receptor (TIE2)

        Angiopoietin/Tie receptor signaling cascades are involved in fundamental angiogenesis events including vascular stabilization and remodeling, as well as recruitment of pericytes...

      • Angiopoietin-2 (ANGPT2)

        Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absen...

      • Arginase-1 (ARG1)

        Arginase-1 is a protein encoded by the ARG1 gene.

      • C-C motif chemokine 17 (CCL17)

        CCL17 (also known as thymus and activation regulated chemokine, TARC) is s a small cytokine belonging to the CC chemokine family. The protein is expressed constitutively in thym...

      • C-C motif chemokine 19 (CCL19)

        CCL19 is a chemokine that upon binding to its receptors CCR10 and CCR7 may play a role in normal lymphocyte recirculation and homing as well as in inflammatory and immunological...

      • C-C motif chemokine 20 (CCL20)

        CCL20 is a chemokine involved in chemotaxis and inflammatory response. Experimental results in animal models of multiple sclerosis suggest that CCL20 may play a critical role in...

      • C-C motif chemokine 23 (CCL23)

        CCL23 is a chemokine with highly chemotactic activity for resting T cells and monocytes. CCL23 has been described to be involved in several inflammation-related diseases such as...

      • C-C motif chemokine 3 (CCL3)

        CCL3, also known as Macrophage inflammatory protein-1? (MIP-1?), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recru...

      • C-C motif chemokine 4 (CCL4 )

        CCL4 (MIP 1-beta) is a chemokine that upon binding to its receptor CCR5 is involved in chemotaxis and inflammatory response, and it is one of the major HIV-suppressive factors p...

      • C-X-C motif chemokine 1 (CXCL1)

        CXCL1 is a chemokine that upon binding to its receptor CXCR2 elicits chemotactic activity on neutrophils and among others is involved in inflammatory response. CXCL1 has been de...

      • C-X-C motif chemokine 10 (CXCL10 )

        CXCL10 (IP-10) is a chemokine that upon binding to its receptor CXCR3 elicits its chemotactic activity on monocytes, T cells, NK cells, and dendritic cells. IP-10 is not constit...

      • C-X-C motif chemokine 11 (CXCL11)

        CXCL11 is a chemokine that upon binding to its receptor CXCR3 elicits chemotactic activity on interleukin-activated T cells and it is also involved in inflammatory response. CXC...

      • C-X-C motif chemokine 13 (CXCL13 )

        C-X-C motif chemokine 13 (CXCL13) also known as B lymphocyte chemoattractant (BLC), is a small cytokine belonging to the CXC chemokine family. CXCL13 and its receptor CXCR5 cont...

      • C-X-C motif chemokine 5 (CXCL5 )

        CXCL5 is a chemokine that upon binding to its receptor CXCR2 elicits chemotactic activity on neutrophils. It is activated upon interleukin-1 and TNF-alpha stimulation. CXCL5 has...

      • C-X-C motif chemokine 9 (CXCL9 )

        CXCL9 (MIG) is a chemokine that upon binding to its receptor CXCR3 elicits chemotactic activity on T cells and is involved in inflammatory response. CXCL9 is not constitutively ...

      • Carbonic anhydrase IX (CAIX)

        CA9 is considered to be one of the best cellular biomarkers of hypoxia. Furthermore, recent studies examining the association between CA9 levels and various clinicopathological ...

      • Caspase-8 (CASP-8 )

        CASP-8 is a cysteine protease that functions as an activator of effector caspases and is involved in apoptosis. In the immune system it is also involved in activation of T, B, a...

      • CD27 antigen (CD27)

        CD27 antigen is a member of the tumor necrosis factor receptor superfamily, and is needed for generation and maintenance of T cell immunity. Via its interactions with ligand CD7...

      • CD40 ligand (CD40-L)

        Soluble CD40 ligand (sCD40L) is contained in platelet granules and thus its presence in the blood is a marker of platelet activation. By interacting with CD40, which is found on...

      • CD40L receptor (CD40)

        CD40 is a member of the TNF-receptor superfamily. It is found on antigen presenting cells where, upon binding of its ligand CD40L, it stimulates them and induces a broad variety...

      • CD70 antigen (CD70)

        CD70 antigen is expressed on highly activated lymphocytes, where it functions as a ligand for CD27. Due to its preferential expression in lymphomas compared to normal lymphocyte...

      • CD83 antigen (CD83)

        May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

      • Cytotoxic and regulatory T-cell molecule (CRTAM)

        Cytotoxic and regulatory T-cell molecule (also known as CD_antigen: CD355) is mainly expressed in cells of the immune system, but also in Purkinje neurons in cerebellum. CRTAM i...

      • Decorin (DCN)

        Decorin is a proteoglycan belonging to the small leucine-rich proteoglycan (SLRP) family. It is a component of connective tissu and binds to type I collagen fibrils. The main fu...

      • Fas antigen ligand (FasL)

        Tumor necrosis factor ligand superfamily member 6 (FASLG), also known as Fas antigen ligand (FasL), induces apoptosis by binding to its receptor and forming the death-inducing s...

      • Fibroblast growth factor 2 (FGF2)

        Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induc...

      • Fractalkine (CX3CL1 )

        CX3CL1 is a chemokine that, when in membrane-bound form promotes adhesion of T cells and monocytes to endothelial cells and, when in soluble form, has a chemotactic function for...

      • Galectin-1 (Gal-1)

        Galectin-1 (Gal-1) belongs to a family of proteins defined by their binding specificity for ?-galactoside sugars, but which unlike most membrane-bound lectins, are soluble prote...

      • Galectin-9 (Gal-9)

        Gal-9 is an S-type lectin. It is strongly overexpressed in Hodgkin's disease tissue and may play a role in the pathogenesis of this disease and/or its consistently associated im...

      • Granzyme A (GZMA)

        Granzyme A (also known as Hanukkah factor) is a protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morpholo...

      • Granzyme B (GZMB)

        Granzyme B (GZMB), also known as Granzyme-2 and human lymphocyte protein, is a serine protease that is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. ...

      • Granzyme H (GZMH)

        Granzyme H (GZMH), also known as cytotoxic serine protease C and cytotoxic T-lymphocyte proteinase, is a chymotrypsin-like serine protease with cytotoxic activity. The enzyme ha...

      • Heme oxygenase 1 (HO-1)

        Heme oxygenase, an essential enzyme in heme catabolism. It cleaves the heme ring to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase. The pr...

      • Hepatocyte growth factor (HGF)

        HGF is a growth, motility and morphogenic factor for a plethora of tissues and cell types and it plays important roles in angiogenesis, tumorogeneis, and tissue regeneration. HG...

      • ICOS ligand (ICOSLG)

        Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and ...

      • Interferon beta (IFN-beta)

        Has antiviral, antibacterial and anticancer activities.

      • Interferon gamma (IFN-gamma)

        IFN-gamma is a cytokine that plays critical roles in innate and adaptive immunity against viral and bacterial infections as well as tumor control. It functions as a stimulator a...

      • Interleukin-1 alpha (IL-1 alpha)

        IL-1 alpha is an interleukin that plays an important role in immune responses. IL-1 alpha is constitutively produced in the epidermis where it plays an important role in mainten...

      • Interleukin-10 (IL10)

        Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

      • Interleukin-12 (IL-12)

        Interleukin-12 is a heterodimeric cytokine encoded by two separate genes, IL-12A and IL-12B. It is involved in the differentiation of naive T cells into Th1 cells, and also stim...

      • Interleukin-12 receptor subunit beta-1 (IL12RB1)

        Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affin...

      • Interleukin-13 (IL-13)

        IL-13 is a cytokine that functions as inducer of matrix of metalloproteinases (MMP) proteins in the airways; induction of these proteinases will result in inhibition of inflamma...

      • Interleukin-18 (IL-18)

        IL-18 is a pro-inflammatory cytokine that in combination with IL-12 induces cell-mediated immunity in the presence of microbial infection, which leads to the release of IFN-gamm...

      • Interleukin-2 (IL-2)

        IL-2 is a cytokine that upon binding to its receptor IL-2R stimulates proliferation of T cells, but it can also stimulate other immune cells. IL-2 plays and important role in T ...

      • Interleukin-21 (IL-21)

        Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. May play a role...

      • Interleukin-33 (IL-33)

        IL-33 has been described as both a pro-inflammatory and immunoregulatory cytokine that can bind two different receptors: IL1RL1 (ST2) and IL1RAP. IL-33 induces production of oth...

      • Interleukin-35 (IL-35)

        Interleukin 35 (IL-35) is an IL-12 family cytokine produced by regulatory, but not effector, T-cells and plays a role in immune suppression. It is a dimeric protein composed of ...

      • Interleukin-4 (IL-4)

        IL-4 has been described as both an anti-inflammatory and a pro-inflammatory cytokine. It activates na?ve T cells to produce Th2 cells, stimulates activated B and T cell proliferat.

      • Interleukin-5 (IL5)

        Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.

      • Interleukin-6 (IL6)

        Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig...

      • Interleukin-7 (IL-7)

        IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stim...

      • Interleukin-8 (IL-8)

        IL-8 is a chemokine that can bind many different cell membrane receptors and function as an important mediator of the immune reaction in the innate immune system response. IL-8 ...

      • Latency-associated peptide transforming growth factor beta-1 (LAP TGF-beta-1)

        TGF-beta-1 is a multifunctional protein that plays important roles in regulation of cell proliferation, differentiation, etc. in many types of cell. It may also play an importan...

      • Lysosome-associated membrane glycoprotein 3 (LAMP3)

        May play a role in dendritic cell function and in adaptive immunity.

      • Macrophage colony-stimulating factor 1 (CSF-1)

        CSF-1 is a cytokine and a growth factor that functions as an inducer of proliferation and differentiation of hematopoietic stem cells to macrophages and monocytes. CSF-1 plays a...

      • Matrix metalloproteinase-12 (MMP-12)

        MMP-12 may be involved in tissue injury and remodeling. Has significant elastolytic activity.

      • Matrix metalloproteinase-7 (MMP-7)

        Extracellular matrix-degrading proteinases are upregulated in atherosclerotic lesions and can contribute to subsequent pathological events. Can be regarded as risk factors, and ...

      • MHC class I polypeptide-related sequence A/B (MIC-A/B)

        MHC class I polypeptide-related sequences A/B are expressed on the cell surface, although unlike canonical class I molecules do not seem to associate with beta-2-microglobulin. ...

      • Monocyte chemotactic protein 1 (MCP-1)

        MCP-1 (CCL2) is a chemokine secreted by monocytes, macrophages, and dendritic cells and it is also anchored to the cell membrane of endothelial cells and activated cleavage by M...

      • Monocyte chemotactic protein 2 (MCP-2)

        MCP-2 (CCL8) is a chemokine that upon binding to its receptors CCR1, CCR2B, and CCR5, elicits a chemotactic activity on many different immune cells that are involved in the infl...

      • Monocyte chemotactic protein 3 (MCP-3)

        MCP-3, also called CCL7, is a chemokine that can bind receptors CCR1, CCR2, and CCR3 and is involved in chemotaxis and inflammatory response. CCL7 has been described to be invol...

      • Monocyte chemotactic protein 4 (MCP-4)

        MCP-4 (CCL13) is a chemokine that upon binding to its receptors CCR2B and CCR3 elicits a chemotactic activity in monocytes, eosinophils, T lymphocytes, and basophils. MCP-4 can ...

      • Natural cytotoxicity triggering receptor 1 (NCR1)

        Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

      • Natural killer cell receptor 2B4 (CD244)

        CD244 (SLAMF4) is a glycoprotein receptor expressed on NK cells, monocytes, and basophils that functions as a modulator of leukocyte activation. Due to the its high polymorphism...

      • Natural killer cells antigen CD94 (KLRD1)

        Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells.

      • Nitric oxide synthase, endothelial (NOS3)

        Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth ...

      • Placenta growth factor (PGF)

        Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1...

      • Platelet-derived growth factor subunit B (PDGF subunit B)

        PDGF subunit B is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta, ...

      • Pleiotrophin (PTN)

        Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells (PubMed:1768439, PubMed:1733956). Binds anaplasti...

      • Pro-epidermal growth factor (EGF)

        EGF is prototypic of a family of growth factors that are derived from membrane-anchored precursors. A wide variety of in vitro and in vivo biological effects have been ascribed ...

      • Programmed cell death 1 ligand 1 (PD-L1)

        PD-L1 is a ligand of the inhibitory receptor PD-1, and together they have an inhibitory function on both microbial and self-reactive T cell responses. It is up-regulated on vari...

      • Programmed cell death 1 ligand 2 (PD-L2)

        PD-1 ligand 2 is a trans-membrane protein that is essential for T-cell proliferation and IFNG production. This function involves a costimulatory signal that is Programmed cell d...

      • Programmed cell death protein 1 (PDCD1)

        Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antig...

      • Stromal cell-derived factor 1 (CXCL12)

        Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intrac...

      • T-cell surface glycoprotein CD4 (CD4)

        CD4 is a glycoprotein found on the surface of immune cells such as T helper cells, functioning as an accessory protein for MHC class-II antigen/T-cell receptor interactions. It ...

      • T-cell surface glycoprotein CD5 (CD5)

        CD5 is a membrane-associated glycoprotein found in B-1 cells where it mitigates the effects of normal tissue proteins. Very few studies have measured circulating forms of CD5 an...

      • T-cell surface glycoprotein CD8 alpha chain (CD8A)

        Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains...

      • T-cell-specific surface glycoprotein CD28 (CD28)

        Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells i...

      • TNF-related apoptosis-inducing ligand (TRAIL)

        TNF-related apoptosis-inducing ligand, TRAIL (also known as TNFSF10) is a cytokine that upon binding to its different receptors can activate apoptosis. However, its activity can...

      • Tumor necrosis factor (Ligand) superfamily, member 12 (TWEAK)

        TWEAK (TNFSF12) is a cytokine that upon binding to its receptor FN14 (TWEAKR) induces multiple cell type-specific signaling pathways that lead to apoptosis, and it also promotes...

      • Tumor necrosis factor (TNF)

        TNF (TNF-alpha) is a cytokine that upon binding to its receptors TNFRSF1A and TNFRSF1B activates NF-kappaB, MAPK, but also in a lower level apoptosis signaling pathways. TNF pla...

      • Tumor necrosis factor ligand superfamily member 14 (TNFSF14 )

        TNFSF14 (LIGHT) is a pro-inflammatory cytokine that plays crucial roles in T cell homing into inflamed tissues and induction of matrix metalloproteinases (MMP) in macrophages. T...

      • Tumor necrosis factor receptor superfamily member 12A (TNFRSF12A)

        Tumor necrosis factor receptor superfamily member 12A (also known as tweak-receptor and CD266) is a type I membrane protein receptor for TNFSF12/TWEAK. It is a weak inducer of a...

      • Tumor necrosis factor receptor superfamily member 21 (TNFRSF21)

        Tumor necrosis factor receptor superfamily member 21 (also known as death receptor 6) is a cell surface receptor of the TNFR superfamily, which activates the JNK and NF-?B pathw...

      • Tumor necrosis factor receptor superfamily member 4 (TNFRSF4 )

        Receptor for TNFSF4/OX40L/GP34. Increasing evidence show that OX40 ligand (OX40L), also known as tumor necrosis factor superfamily member 4 (TNFSF4), plays an important role in ...

      • Tumor necrosis factor receptor superfamily member 9 (TNFRSF9)

        TNFRSF9 is a member of the tumor necrosis factor receptor family and is possibly active during T cell activation. It can be found in several cell types at sites of inflammation....

      • Vascular endothelial growth factor A (VEGF-A)

        VEGF-A is a growth factor that upon binding to its receptors (VEGFR1 and VEGFR2) and to heparan sulfate and heparin, induces endothelial cell proliferation, promotes cell migrat...

      • Vascular endothelial growth factor C (VEGFC)

        Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May f...

      • Vascular endothelial growth factor receptor 2 (VEGFR-2)

        Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascula...