• Proseek Multiplex Inflammation I

    Towards a better understanding of inflammation and personalized medicine

    Inf-newProseek® Multiplex Inflammation I96×96 is a high-throughput, multiplex immunoassay enabling analysis of 92 inflammation-related protein biomarkers across 96 samples simultaneously. This high level of multiplexing is achieved without any compromise on data quality, thanks to our proprietary Proximity Extension Assay (PEA) technology

    This panel represents the most comprehensive ready-to-use kit of inflammatory-related analytes on the market, enabling investigation of protein signatures with high efficiency and robustness and accelerates the speed of finding new and relevant human protein biomarkers related to inflammation. The panel is compiled to detect a selection of already established as well as exploratory biomarkers within the inflammation research field. The content of the panel has been designed in close collaboration with experts within various disease areas such as rheumatoid arthritis, Crohn’s disease, ulcerative colitis, neuro inflammation, and respiratory diseases, and it is well suited to discerning protein profiles in clinically relevant samples.

    An overview of how the 92 biomarker assays in the panel are classified according to Biological Process, Disease Area, Tissue Expression and Protein Class (based on widely used public-access bioinformatic databases, including Uniprot, Human Protein Atlas, Gene Ontology (GO) and DisGeNET) can be seen in the charts below. These charts are interactive: click on a chart-bar or pie-segment to see the list of biomarkers in that classification. Each item in the list links to the detailed biomarker page with validation data.

    Biological Process

    Disease Area

    Tissue Expression

    Protein Class

    For optimal assay performance, each immunoassay in the panel has been assessed in terms of specificity, precision, sensitivity, dynamic range, matrix effects and interference in biological material.

    A comprehensive list of all protein biomarkers that can be measured using the currently available Proseek Multiplex panels can be seen on the biomarker library page.

    Note: A printable list of the biomarker assays available in this panel, as well as a full data validation document, can be downloaded from our Document download center >>

    If you prefer not to run the assay yourself, let our experts here at Olink do this for you via our Analysis Service.

    The biomarker pages linked to below include calibrator curves that show the performance of each assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens, with data presented as Normalized Protein eXpression (NPX) values plotted against protein concentration (in pg/mL). Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please view the FAQ.

  • Search all our biomarkers

      Biomarkers in Proseek Multiplex Inflammation

      • Adenosine Deaminase (ADA)

        ADA is a key enzyme of the purine metabolism and it is involved in the breakdown of adenosine into nucleic acids. In humans it is primarily involved in the development and maint...

      • Artemin (ARTN)

        Artermin is a neurotrophic factor from the GDNF family of ligands inside the TGF beta superfamily. Artemin is a suitable protein for performing exploratory analysis for the disc...

      • Axin-1 (AXIN1)

        Axin-1 is a protein that functions as a negative regulator in the Wnt signaling pathway. Axin-1 is a suitable protein for performing exploratory analysis for the discovery of ne...

      • Beta-nerve growth factor (Beta-NGF)

        Beta-NGF is a neurotrophic factor with an important role in neuron survival and it has been studied in various neurological disease conditions. Beta-NGF plays also a role as a s...

      • Brain-derived neurotrophic factor (BDNF)

        BDNF is a neurotrophic factor and a myokine which has been studied in various neurological and metabolic disease conditions. BDNF is also produced by inflammatory cells and has ...

      • C-C motif chemokine 19 (CCL19)

        CCL19 is a chemokine that upon binding to its receptors CCR10 and CCR7 may play a role in normal lymphocyte recirculation and homing as well as in inflammatory and immunological...

      • C-C motif chemokine 20 (CCL20)

        CCL20 is a chemokine involved in chemotaxis and inflammatory response. Experimental results in animal models of multiple sclerosis suggest that CCL20 may play a critical role in...

      • C-C motif chemokine 23 (CCL23)

        CCL23 is a chemokine with highly chemotactic activity for resting T cells and monocytes. CCL23 has been described to be involved in several inflammation-related diseases such as...

      • C-C motif chemokine 25 (CCL25)

        CCL25 is a chemokine that upon binding to its receptor CCR9 is involved in chemotaxis and inflammatory response. CCL25 is highly expressed in the thymus and small intestine and ...

      • C-C motif chemokine 28 (CCL28)

        CCL28 is a chemokine that upon binding to its receptors CCR10 and CCR3 exerts its chemotactic activity on T and B cells, and eosinophils and together they play an important role...

      • C-C motif chemokine 3 (CCL3)

        CCL3, also known as Macrophage inflammatory protein-1? (MIP-1?), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recru...

      • C-C motif chemokine 4 (CCL4 )

        CCL4 (MIP 1-beta) is a chemokine that upon binding to its receptor CCR5 is involved in chemotaxis and inflammatory response, and it is one of the major HIV-suppressive factors p...

      • C-X-C motif chemokine 1 (CXCL1)

        CXCL1 is a chemokine that upon binding to its receptor CXCR2 elicits chemotactic activity on neutrophils and among others is involved in inflammatory response. CXCL1 has been de...

      • C-X-C motif chemokine 10 (CXCL10 )

        CXCL10 (IP-10) is a chemokine that upon binding to its receptor CXCR3 elicits its chemotactic activity on monocytes, T cells, NK cells, and dendritic cells. IP-10 is not constit...

      • C-X-C motif chemokine 11 (CXCL11)

        CXCL11 is a chemokine that upon binding to its receptor CXCR3 elicits chemotactic activity on interleukin-activated T cells and it is also involved in inflammatory response. CXC...

      • C-X-C motif chemokine 5 (CXCL5 )

        CXCL5 is a chemokine that upon binding to its receptor CXCR2 elicits chemotactic activity on neutrophils. It is activated upon interleukin-1 and TNF-alpha stimulation. CXCL5 has...

      • C-X-C motif chemokine 6 (CXCL6)

        CXCL6 is a chemokine that upon binding to its receptors CXCR1 and CXCR2 elicits chemotactic activity on neutrophilic granulocytes. CXCL6 has been described to be involved in inf...

      • C-X-C motif chemokine 9 (CXCL9 )

        CXCL9 (MIG) is a chemokine that upon binding to its receptor CXCR3 elicits chemotactic activity on T cells and is involved in inflammatory response. CXCL9 is not constitutively ...

      • Caspase-8 (CASP-8 )

        CASP-8 is a cysteine protease that functions as an activator of effector caspases and is involved in apoptosis. In the immune system it is also involved in activation of T, B, a...

      • CD40L receptor (CD40)

        CD40 is a member of the TNF-receptor superfamily. It is found on antigen presenting cells where, upon binding of its ligand CD40L, it stimulates them and induces a broad variety...

      • CUB domain-containing protein 1 (CDCP1)

        CDCP1 is a transmembrane glycoprotein involved in negative regulation of cell adhesion. CDCP1 is a suitable protein for performing exploratory analysis for the discovery of new ...

      • Cystatin D (CST5)

        CST5 is a cysteine proteinase inhibitor found in human saliva and tear fluid and may play a role in controlling proteolytic activity during inflammatory processes. CST5 is a sui...

      • Delta and Notch-like epidermal growth factor-related receptor (DNER)

        DNER is an activator of the NOTCH1 pathway. DNER is a suitable protein for performing exploratory analysis for the discovery of new inflammation-related biomarkers.

      • Eotaxin (CCL11)

        In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.

      • Eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1)

        EIF4EBP1 is a regulator of protein translation. EIF4BP1 is a suitable protein for performing exploratory analysis for the discovery of new inflammation-related biomarkers.

      • Fibroblast growth factor 19 (FGF-19)

        FGF-19 is a hormone and growth factor involved in regulation of bile acid synthesis. FGF-19 is also involved in processes of the adaptive and innate immune systems. FGF-19 has b...

      • Fibroblast growth factor 21 (FGF21)

        Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the ...

      • Fibroblast growth factor 23 (FGF-23)

        FGF-23 is a growth factor secreted by osteocytes in response to calcitriol (the hormonally active form of vitamin D), and it is involved in the regulation of phosphate concentra...

      • Fibroblast growth factor 5 (FGF-5)

        FGF-5 is a growth factor involved in regulation of cellular proliferation and differentiation, and it is well known as an inhibitor of hair elongation. FGF-5 is a suitable prote...

      • Fms-related tyrosine kinase 3 ligand (Flt3L)

        Flt3L is a cytokine that is involved in the mobilization and differentiation of hematopoietic stem cells and have a crucial role in dendritic cell development. Flt3L has been de...

      • Fractalkine (CX3CL1 )

        CX3CL1 is a chemokine that, when in membrane-bound form promotes adhesion of T cells and monocytes to endothelial cells and, when in soluble form, has a chemotactic function for...

      • Glial cell line-derived neurotrophic factor (GDNF)

        hGDNF is a neurotrophic factor that is involved in neuron survival and regeneration, and has been suggested for potential treatment of Parkinson?s disease. hGDNF has been descri...

      • Hepatocyte growth factor (HGF)

        HGF is a growth, motility and morphogenic factor for a plethora of tissues and cell types and it plays important roles in angiogenesis, tumorogeneis, and tissue regeneration. HG...

      • Interferon gamma (IFN-gamma)

        IFN-gamma is a cytokine that plays critical roles in innate and adaptive immunity against viral and bacterial infections as well as tumor control. It functions as a stimulator a...

      • Interleukin-1 alpha (IL-1 alpha)

        IL-1 alpha is an interleukin that plays an important role in immune responses. IL-1 alpha is constitutively produced in the epidermis where it plays an important role in mainten...

      • Interleukin-10 (IL10)

        Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

      • Interleukin-10 receptor subunit alpha (IL-10RA)

        IL-10RA is one of the subunits of cytokine receptor IL-10R that, together with IL-10RB has been shown to be required for IL-10-induced signal transduction. Thus, IL-10RA plays a...

      • Interleukin-10 receptor subunit beta (IL-10RB)

        IL-10RB is a one the subunits of the cytokine receptor IL-10R that, together with IL-10RA has been shown to be required for IL-10-induced signal transduction. Thus, IL-10RB play...

      • Interleukin-12 subunit beta (IL-12B)

        IL-12B is a cytokine that can heterodimerize with both IL-12A and IL-23A to form IL-12 (p70) and IL-23 respectively. It can also be found as an homodimer. IL-12 stimulates the p...

      • Interleukin-13 (IL-13)

        IL-13 is a cytokine that functions as inducer of matrix of metalloproteinases (MMP) proteins in the airways; induction of these proteinases will result in inhibition of inflamma...

      • Interleukin-15 receptor subunit alpha (IL-15RA)

        IL-15RA is one of the subunits of the cytokine receptor IL-15R, which is composed of two additional subunits (IL-2RB and IL-2RG wich are shared with IL-2R). IL-15RA subunit has ...

      • Interleukin-17A (IL-17A)

        IL-17A is a pro-inflammatory cytokine that is induced by IL-23 in delayed-type reactions. It plays an important role in inducing production of other cytokines, chemokines, and p...

      • Interleukin-17C (IL-17C)

        IL-17C is a pro-inflammatory cytokine that acts synergistically with IL-22, TNF, and IL-1B in the induction of anti-microbial peptides, but it can also have a protective functio...

      • Interleukin-18 (IL-18)

        IL-18 is a pro-inflammatory cytokine that in combination with IL-12 induces cell-mediated immunity in the presence of microbial infection, which leads to the release of IFN-gamm...

      • Interleukin-18 receptor 1 (IL-18R1)

        IL-18R1 is one of the subunits of the cytokine receptor IL-18R, and it plays an essential role in IL-18 mediated signal transduction in immunity and inflammation. IL-12 and IFN-...

      • Interleukin-2 (IL-2)

        IL-2 is a cytokine that upon binding to its receptor IL-2R stimulates proliferation of T cells, but it can also stimulate other immune cells. IL-2 plays and important role in T ...

      • Interleukin-2 receptor subunit beta (IL-2RB)

        IL-2RB is one of the subunits of the cytokine receptor IL-2R and upon binding of its ligand IL-2 it plays a role in receptor mediated endocytosis and signal transduction for the...

      • Interleukin-20 (IL-20)

        IL-20 is a pro-inflammatory cytokine that upon binding to its heterodimeric receptor acts on keratinocytes and other epithelial cells activating the STAT3 signaling pathway and ...

      • Interleukin-20 receptor subunit alpha (IL-20RA)

        IL-20RA is one of the subunits of two heterodimeric cytokine receptors. Together with IL-20RB it functions as a receptor for IL-19, IL-20, and IL-24. Together with IL-10RB it fu...

      • Interleukin-22 receptor subunit alpha-1 (IL-22 RA1)

        IL-22RA1 is one of the subunits of two heterodimeric cytokine receptors. Together with IL-10RB it functions as a receptor for IL-20. Together with IL-20RB it functions as a rece...

      • Interleukin-24 (IL-24)

        IL-24 is a pro-inflammatory cytokine that upon binding to any of its two heterodimeric receptors IL-20R1/IL-20R2 and IL-22R1/IL-20R2 functions as an activator of STAT3 signaling...

      • Interleukin-33 (IL-33)

        IL-33 has been described as both a pro-inflammatory and immunoregulatory cytokine that can bind two different receptors: IL1RL1 (ST2) and IL1RAP. IL-33 induces production of oth...

      • Interleukin-4 (IL-4)

        IL-4 has been described as both an anti-inflammatory and a pro-inflammatory cytokine. It activates na?ve T cells to produce Th2 cells, stimulates activated B and T cell proliferat.

      • Interleukin-5 (IL5)

        Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.

      • Interleukin-6 (IL6)

        Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig...

      • Interleukin-7 (IL-7)

        IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stim...

      • Interleukin-8 (IL-8)

        IL-8 is a chemokine that can bind many different cell membrane receptors and function as an important mediator of the immune reaction in the innate immune system response. IL-8 ...

      • Latency-associated peptide transforming growth factor beta-1 (LAP TGF-beta-1)

        TGF-beta-1 is a multifunctional protein that plays important roles in regulation of cell proliferation, differentiation, etc. in many types of cell. It may also play an importan...

      • Leukemia inhibitory factor (LIF)

        LIF is a cytokine that upon binding to its receptor LIF-R elicits its function as an activator of the JAK/STAT and MAPK signaling pathways that leads to inhibition of cell diffe...

      • Leukemia inhibitory factor receptor (LIF-R)

        LIF-R is one of the subunits of the LIF receptor, and together with gp130 activates JAK/STAT and MAPK signaling pathways upon stimulation by LIF cytokine. LIF-R has been describ...

      • Macrophage colony-stimulating factor 1 (CSF-1)

        CSF-1 is a cytokine and a growth factor that functions as an inducer of proliferation and differentiation of hematopoietic stem cells to macrophages and monocytes. CSF-1 plays a...

      • Matrix metalloproteinase-1 (MMP-1)

        MMP-1 is a protease which function is the cleavage of several types of collagens and thus, it is involved in the degradation of extracellular matrix in both normal physiological...

      • Matrix metalloproteinase-10 (MMP-10)

        MMP-10 (SL-2) is a protease which functions are activation of collagenases and cleavage of fibronectin and several types of collagens and gelatins. Thus, MMP-10 is involved in t...

      • Monocyte chemotactic protein 1 (MCP-1)

        MCP-1 (CCL2) is a chemokine secreted by monocytes, macrophages, and dendritic cells and it is also anchored to the cell membrane of endothelial cells and activated cleavage by M...

      • Monocyte chemotactic protein 2 (MCP-2)

        MCP-2 (CCL8) is a chemokine that upon binding to its receptors CCR1, CCR2B, and CCR5, elicits a chemotactic activity on many different immune cells that are involved in the infl...

      • Monocyte chemotactic protein 3 (MCP-3)

        MCP-3, also called CCL7, is a chemokine that can bind receptors CCR1, CCR2, and CCR3 and is involved in chemotaxis and inflammatory response. CCL7 has been described to be invol...

      • Monocyte chemotactic protein 4 (MCP-4)

        MCP-4 (CCL13) is a chemokine that upon binding to its receptors CCR2B and CCR3 elicits a chemotactic activity in monocytes, eosinophils, T lymphocytes, and basophils. MCP-4 can ...

      • Natural killer cell receptor 2B4 (CD244)

        CD244 (SLAMF4) is a glycoprotein receptor expressed on NK cells, monocytes, and basophils that functions as a modulator of leukocyte activation. Due to the its high polymorphism...

      • Neurotrophin-3 (NT-3)

        NT-3 is a neurotrophic factor in the NGF-beta family that have important functions promoting the survival and differentiation of neurons as well as neurogenesis and formation of...

      • Neurturin (NRTN)

        Neurturin is a neurotrophic factor in the BDNF subfamily (TGF-beta family) that may play an important role in regulating the development and maintenance of the CNS, and possibly...

      • Oncostatin-M (OSM)

        OSM has been described as both a pro- and an anti-inflammatory cytokine that plays an important role in regulation of cytokine production from endothelial cells in inflammation,...

      • Osteoprotegerin (OPG)

        OPG is a decoy receptor of cytokines TNFSF11 (RANKL) and possibly TNFSF10 (TRAIL) and belongs to the TNF receptor superfamily. OPG is up-regulated by estrogens and increasing ca...

      • Programmed cell death 1 ligand 1 (PD-L1)

        PD-L1 is a ligand of the inhibitory receptor PD-1, and together they have an inhibitory function on both microbial and self-reactive T cell responses. It is up-regulated on vari...

      • Protein S100-A12 (EN-RAGE )

        EN-RAGE is a calcium-, zinc-, and copper-binding protein involved in regulation of inflammatory processes and immune response. EN-RAGE has been described to be involved in sever...

      • Signaling lymphocytic activation molecule (SLAMF1)

        SLAMF1 (SLAM) is a glycoprotein and self-ligand receptor on the cell membrane of activated T and B cells, macrophages, and dendritic cells, that plays and important role in cont...

      • SIR2-like protein 2 (SIRT2)

        SIRT2 is a NAD-dependent protein deacetylase that may play a role in Alzheimer?s disease. SIRT2 is a suitable protein for performing exploratory analysis for the discovery of ne...

      • STAM-binding protein (STAMBP)

        STAMBP is a zinc metalloprotease involved in endosomal deubiquitination. It plays roles in regulation of receptor-mediated endocytosis, and signal-transduction for cell growth a...

      • Stem cell factor (SCF)

        SCF (c-Kit-ligand) is a cytokine that plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. SCF is up-regulated under inflammatory conditions and by stim...

      • Sulfotransferase 1A1 (ST1A1)

        ST1A1 (SULT1A1) is a sulfotransferase that catalyzes the sulfate conjugation of catecholamines, estrogen, phenolic drugs, and neurotransmitters. Thus, ST1A1 plays an important r...

      • T cell surface glycoprotein CD6 isoform (CD6)

        CD6 is a membrane-associated glycoprotein involved in T cell activation. CD6 is a suitable protein for performing exploratory analysis for the discovery of new inflammation-rela...

      • T-cell surface glycoprotein CD5 (CD5)

        CD5 is a membrane-associated glycoprotein found in B-1 cells where it mitigates the effects of normal tissue proteins. Very few studies have measured circulating forms of CD5 an...

      • Thymic stromal lymphopoietin (TSLP)

        TSLP is a cytokine that upon binding to the heterodimeric receptor complex formed by CRLF2 and IL-7Ralpha, induces the JAK-STAT signaling pathway. It is involved in the activati...

      • TNF-beta (TNFB)

        TNFB (LT-alpha) is a cytokine that forms heterotrimers with LT-beta (TNF-C), and upon binding to the receptor LTBR (TNFRSF3) mediates a large variety of inflammatory, immunostim...

      • TNF-related activation-induced cytokine (TRANCE)

        TRANCE (TNFSF11, RANKL, OPGL) is a cytokine in the TNF family that upon binding to its receptors RANK and OPG, plays important roles in dendritic cell maturation and survival, r...

      • TNF-related apoptosis-inducing ligand (TRAIL)

        TNF-related apoptosis-inducing ligand, TRAIL (also known as TNFSF10) is a cytokine that upon binding to its different receptors can activate apoptosis. However, its activity can...

      • Transforming growth factor alpha (TGF-alpha)

        TGF-alpha is a growth factor produced in macrophages, brain cells, and keratinocytes, and upon its receptor EGFR it functions as an inducer of epithelial development. TGF-alpha ...

      • Tumor necrosis factor (Ligand) superfamily, member 12 (TWEAK)

        TWEAK (TNFSF12) is a cytokine that upon binding to its receptor FN14 (TWEAKR) induces multiple cell type-specific signaling pathways that lead to apoptosis, and it also promotes...

      • Tumor necrosis factor (TNF)

        TNF (TNF-alpha) is a cytokine that upon binding to its receptors TNFRSF1A and TNFRSF1B activates NF-kappaB, MAPK, but also in a lower level apoptosis signaling pathways. TNF pla...

      • Tumor necrosis factor ligand superfamily member 14 (TNFSF14 )

        TNFSF14 (LIGHT) is a pro-inflammatory cytokine that plays crucial roles in T cell homing into inflamed tissues and induction of matrix metalloproteinases (MMP) in macrophages. T...

      • Tumor necrosis factor receptor superfamily member 9 (TNFRSF9)

        TNFRSF9 is a member of the tumor necrosis factor receptor family and is possibly active during T cell activation. It can be found in several cell types at sites of inflammation....

      • Urokinase-type plasminogen activator (uPA)

        uPA is a serine protease that activates plasminogen to give the serine protease plasmin. This activation leads to a proteolysis cascade that ultimately is important for the degr...

      • Vascular endothelial growth factor A (VEGF-A)

        VEGF-A is a growth factor that upon binding to its receptors (VEGFR1 and VEGFR2) and to heparan sulfate and heparin, induces endothelial cell proliferation, promotes cell migrat...