• Proseek Multiplex Metabolism

    Casting a broader net to further explore the complexity of the human proteome

    Complementing and expanding on our previous portfolio of disease area-focused biomarker panels, our exploratory series offers scientists the chance to cast a wider net in the quest to identify new biomarkers and relevant protein signatures, offering many new assays focused on important biological processes with wide-ranging clinical relevance.

    The Metabolism panel offers simultaneous analysis of 92 protein biomarkers using just 1 µL of biological sample, and all assays are subject to Olink’s strict technical validation and QC control procedures. The selection of protein biomarker assays is designed to provide the optimal dynamic range, as well as to focus on proteins with relevance for immune response processes. Categorization of the proteins included in the panel was carried out via referral to widely used public-access bioinformatic databases, including Uniprot, Human Protein Atlas,  Gene Ontology (GO) and DisGeNET. The assays in this panel include proteins involved in key biological processes such as cellular metabolic processes, cell surface receptor signaling pathways, regulation of phosphorylation and cell adhesion.

    An alphabetical list of the 92 proteins with links to the individual biomarker pages containing additional information and assay validation data can be found at the bottom of this page.

    An overview of how the 92 biomarker assays in the panel are classified according to Biological Process, Disease Area, Tissue Expression and Protein Class can be seen in the charts below. These charts are interactive: click on a chart-bar or pie-segment to see the list of biomarkers in that classification. Each item in the list links to the detailed biomarker page with validation data.

    Biological Process

    Disease Area

    Tissue Expression

    Protein Class

     

    Please Note: the Metabolism panel uses a 1:10 dilution of sample, whereas our technical validation assays are performed in vitro using recombinant analytes. Technical validation data reported on the biomarker pages below, and in the data validation document for this panel are based on these in vitro assays. A multiplication factor of 10 should therefore be taken into consideration when comparing the addressable biological concentration to the in vitro validation data. (Download the sample dilution guide document for this panel here)

    A comprehensive list of all protein biomarkers that can be measured using the currently available biomarker panels can be seen on the biomarker library page.

    Note: A printable list of the biomarker assays available in this panel, as well as a full data validation document, can be downloaded from our Document download center >>

    If you prefer not to run the assay yourself, let our experts here at Olink do this for you via our Analysis Service.

    The biomarker pages linked to below include calibrator curves that show the performance of each assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens, with data presented as Normalized Protein eXpression (NPX) values plotted against protein concentration (in pg/mL). Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please view the FAQ.

  • Search all our biomarkers

      Biomarkers in Proseek Multiplex Metabolism

      • Adenosylhomocysteinase (AHCY)

        Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the co...

      • Adhesion G protein-coupled receptor E2 (ADGRE2)

        Cell surface receptor that binds to the chondroitin sulfate moiety of glycosaminoglycan chains and promotes cell attachment. Promotes granulocyte chemotaxis, degranulation and a...

      • Adhesion G-protein coupled receptor G2 (ADGRG2)

        Orphan receptor. Could be involved in a signal transduction pathway controlling epididymal function and male fertility. May regulate fluid exchange within epididymis.

      • Amyloid-like protein 1 (APLP1)

        May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarit...

      • Angiopoietin-2 (ANGPT2)

        Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absen...

      • Angiopoietin-related protein 1 (ANGPTL1)

        Angiopoietin-related protein 1 is a protein encoded by the ANGPTL1 gene.

      • Angiopoietin-related protein 7 (ANGPTL7)

        Angiopoietin-related protein 7 is a protein encoded by the ANGPTL7 gene

      • Annexin A11 (ANXA11)

        Binds specifically to calcyclin in a calcium-dependent manner (By similarity). Required for midbody formation and completion of the terminal phase of cytokinesis.

      • Annexin A4 (ANXA4)

        Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.

      • Appetite-regulating hormone (GHRL)

        Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induc...

      • Arginase-1 (ARG1)

        Arginase-1 is a protein encoded by the ARG1 gene.

      • Aromatic-L-amino-acid decarboxylase (DDC)

        Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.

      • B-cell antigen receptor complex-associated protein beta chain (CD79B)

        Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the...

      • C-type lectin domain family 5 member A (CLEC5A)

        Functions as a positive regulator of osteoclastogenesis. Cell surface receptor that signals via TYROBP. Regulates inflammatory responses. Acts as a key regulator of synovial inj...

      • Cadherin-2 (CDH2)

        Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to...

      • Cadherin-related family member 5 (CDHR5)

        Intermicrovillar adhesion molecule that forms, via its extracellular domain, calcium-dependent heterophilic complexes with CDHR2 on adjacent microvilli. Thereby, controls the pa...

      • Calsyntenin-2 (CLSTN2)

        May modulate calcium-mediated postsynaptic signals.

      • Carbonic anhydrase 13 (CA13)

        Reversible hydration of carbon dioxide.

      • Catechol O-methyltransferase (COMT)

        Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroacti...

      • Cathepsin O (CTSO)

        Proteolytic enzyme possibly involved in normal cellular protein degradation and turnover.

      • CD2-associated protein (CD2AP)

        Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. In collaboration with CBLC, modulates the rate of RET turnover and may act as regulatory...

      • Chordin-like protein 2 (CHRDL2)

        May inhibit BMPs activity by blocking their interaction with their receptors. Has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal c...

      • Clusterin-like protein 1 (CLUL1)

        Clusterin-like protein 1 is a protein encoded by the CLUL1 gene

      • Coiled-coil domain-containing protein 80 (CCDC80)

        Promotes cell adhesion and matrix assembly.

      • Crk-like protein (CRKL)

        May mediate the transduction of intracellular signals.

      • CXADR-like membrane protein (CLMP)

        May be involved in the cell-cell adhesion. May play a role in adipocyte differentiation and development of obesity. Is required for normal small intestine development.

      • Diablo homolog, mitochondrial (DIABLO)

        Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibi...

      • Dihydropteridine reductase (QDPR)

        The product of this enzyme, tetrahydrobiopterin (BH-4), is an essential cofactor for phenylalanine, tyrosine, and tryptophan hydroxylases.

      • Dipeptidyl peptidase 2 (DPP7)

        Plays an important role in the degradation of some oligopeptides.

      • Disabled homolog 2 (DAB2)

        Adapter protein that functions as clathrin-associated sorting protein (CLASP) required for clathrin-mediated endocytosis of selected cargo proteins. Can bind and assemble clathr...

      • DNA-(apurinic or apyrimidinic site) lyase (APEX1)

        Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcrip...

      • Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5)

        Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic ret...

      • Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 (ENPP7)

        Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity.

      • Eosinophil cationic protein (RNASE3)

        Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic...

      • Fc receptor-like protein 1 (FCRL1)

        May function as an activating coreceptor in B-cells. May function in B-cells activation and differentiation.

      • Fructose-1,6-bisphosphatase 1 (FBP1)

        Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a r...

      • Galanin peptides (GAL)

        Endocrine hormone of the central and peripheral nervous systems that binds and activates the G protein-coupled receptors GALR1, GALR2, and GALR3. This small neuropeptide may reg...

      • Gamma-enolase (ENO2)

        Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neuron...

      • Glutaredoxin-1 (GLRX)

        Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.

      • GRB2-related adapter protein 2 (GRAP2)

        Interacts with SLP-76 to regulate NF-AT activation. Binds to tyrosine-phosphorylated shc.

      • Hepatoma-derived growth factor (HDGF)

        Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.

      • Inactive tyrosine-protein kinase transmembrane receptor ROR1 (ROR1)

        Has very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo. May act as a receptor for wnt ligand WNT5A which may result in the inhibition of ...

      • Insulin-like growth factor-binding protein-like 1 (IGFBPL1)

        IGF-binding proteins prolong the half-life of IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs in cell culture. They alter the in...

      • Integrin beta-7 (ITGB7)

        Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (...

      • Kallikrein-10 (KLK10)

        Has a tumor-suppressor role for NES1 in breast and prostate cancer.

      • Kynurenine--oxoglutarate transaminase 1 (KYAT1)

        Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Metabolizes the cysteine conjugates of certain halogenated alk...

      • Large proline-rich protein BAG6 (BAG6)

        Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of...

      • Leucine-rich repeats and immunoglobulin-like domains protein 1 (LRIG1)

        Acts as a feedback negative regulator of signaling by receptor tyrosine kinases, through a mechanism that involves enhancement of receptor ubiquitination and accelerated intrace...

      • Leukocyte immunoglobulin-like receptor subfamily A member 5 (LILRA5)

        May play a role in triggering innate immune responses. Does not seem to play a role for any class I MHC antigen recognition.

      • Low-density lipoprotein receptor-related protein 11 (LRP11)

        Low-density lipoprotein receptor-related protein 11 is a protein encoded by the LRP11 gene.

      • Lysophosphatidic acid phosphatase type 6 (ACP6)

        Hydrolyzes lysophosphatidic acid (LPA) containing a medium length fatty acid chain to the corresponding monoacylglycerol. Has highest activity with lysophosphatidic acid contain...

      • Meprin A subunit beta (MEP1B)

        Membrane metallopeptidase that sheds many membrane-bound proteins. Exhibits a strong preference for acidic amino acids at the P1' position. Known substrates include: FGF19, VGFA...

      • Meteorin-like protein (METRNL)

        Hormone induced following exercise or cold exposure that promotes energy expenditure. Induced either in the skeletal muscle after exercise or in adipose tissue following cold ex...

      • Multiple coagulation factor deficiency protein 2 (MCFD2)

        The MCFD2-LMAN1 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins. Plays a role in the secretion of coagulation factors.

      • N-terminal prohormone brain natriuretic peptide (NT-proBNP)

        The N-terminal of the prohormone brain natriuretic peptide (NT-proBNP), also commonly abbreviated BNPT, is a 76 amino acid N-terminal inactive protein that is cleaved from proBN...

      • NAD kinase (NADK)

        NAD kinase is a protein encoded by the NADK gene

      • Nectin-2 (NECTIN2)

        Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell...

      • Neural proliferation differentiation and control protein 1 (NPDC1)

        Suppresses oncogenic transformation in neural and non-neural cells and down-regulates neural cell proliferation. Might be involved in transcriptional regulation (By similarity).

      • Neuronal pentraxin receptor (NPTXR)

        May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory ...

      • Nodal modulator 1 (NOMO1)

        May antagonize Nodal signaling.

      • Paired immunoglobulin-like type 2 receptor beta (PILRB)

        Paired receptors consist of highly related activating and inhibitory receptors and are widely involved in the regulation of the immune system. PILRB is thought to act as a cellu...

      • Peptidyl-prolyl cis-trans isomerase FKBP4 (FKBP4)

        Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a r...

      • Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1)

        Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Prom...

      • Pro-cathepsin H (CTSH)

        Important for the overall degradation of proteins in lysosomes.

      • Protein FAM3C (FAM3C)

        May be involved in retinal laminar formation. Promotes epithelial to mesenchymal transition.

      • Protein phosphatase inhibitor 2 (PPP1R2)

        Inhibitor of protein-phosphatase 1.

      • Protein S100-P (S100P)

        May function as calcium sensor and contribute to cellular calcium signaling. In a calcium-dependent manner, functions by interacting with other proteins, such as EZR and PPP5C, ...

      • Regenerating islet-derived protein 4 (REG4)

        Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammator...

      • Reticulon-4 receptor (RTN4R)

        Receptor for RTN4, OMG and MAG. Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton. Mediates axonal growth inhibition and may play a ro...

      • Retinal dehydrogenase 1 (ALDH1A1)

        Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity).

      • Ribosyldihydronicotinamide dehydrogenase [quinone] (NQO2)

        The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic proc...

      • Scavenger receptor cysteine-rich domain-containing group B protein (SSC4D)

        Scavenger receptor cysteine-rich domain-containing group B protein is encoded by the SSC4D gene.

      • Sclerostin (SOST)

        Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.

      • Semaphorin-3F (SEMA3F)

        May play a role in cell motility and cell adhesion.

      • Serpin B6 (SERPINB6)

        May be involved in the regulation of serine proteinases present in the brain or extravasated from the blood (By similarity). Inhibitor of cathepsin G, kallikrein-8 and thrombin....

      • Serpin B8 (SERPINB8)

        Serpin B8 is a protein encoded by the SERPINB8 gene.

      • Sialic acid-binding Ig-like lectin 7 (SIGLEC7)

        Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosi...

      • Sialomucin core protein 24 (CD164)

        Sialomucin that may play a key role in hematopoiesis by facilitating the adhesion of CD34(+) cells to the stroma and by negatively regulating CD34(+)CD38(lo/-) cell proliferatio...

      • Soluble calcium-activated nucleotidase 1 (CANT1)

        Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lo...

      • Sulfatase-modifying factor 2 (SUMF2)

        Lacks formyl-glycine generating activity and is unable to convert newly synthesized inactive sulfatases to their active form. Inhibits the activation of sulfatases by SUMF1.

      • Synaptosomal-associated protein 23 (SNAP23)

        Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.

      • Syndecan-4 (SDC4)

        Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed:22660413).

      • T-cell surface glycoprotein CD1c (CD1C)

        Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

      • Thimet oligopeptidase (THOP1)

        Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation. Able to degrade the beta-amyloid precursor protein an...

      • Thioredoxin domain-containing protein 5 (TXNDC5)

        Possesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide-isomerase deficiency in yeast (By similarity).

      • Thymidine phosphorylase (TYMP)

        May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on en...

      • Thyrotropin subunit beta (TSHB)

        Indispensable for the control of thyroid structure and metabolism.

      • Trefoil factor 2 (TFF2)

        Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel ...

      • Tubulointerstitial nephritis antigen-like (TINAGL1)

        May be implicated in the adrenocortical zonation and in mechanisms for repressing the CYP11B1 gene expression in adrenocortical cells. This is a non catalytic peptidase C1 famil...

      • Tyrosine-protein kinase receptor TYRO3 (TYRO3)

        Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiolog...

      • Ubiquitin carboxyl-terminal hydrolase 8 (USP8)

        Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Convert...

      • Versican core protein (VCAN)

        May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. B...