• Proseek Multiplex Oncology II

    Towards a better understanding of cancer and personalized medicine

    Onc-PptCancer remains one of the major causes of death and continues to require massive global research efforts. The ability to discover and validate more cancer-related protein biomarkers will be instrumental in addressing this huge healthcare challenge – paving the way for better understanding, diagnosis, and personalized treatment for cancer patients.

    Proseek® Multiplex Oncology II96×96 is a high-throughput, multiplex immunoassay enabling analysis of 92 inflammation-related protein biomarkers across 96 samples simultaneously. This high level of multiplexing is achieved without any compromise on data quality, thanks to our proprietary Proximity Extension Assay (PEA) technology

    The panel comprises 92 putative cancer-related human proteins that participate in biological mechanisms that are central to the initiation and progression of cancer, e.g. angiogenesis, cell-cell signaling, cell-cycle control, and inflammation. For optimal assay performance, each analyte in the panel has been assessed in terms of sample material, specificity, precision, sensitivity, dynamic range, matrix effects and interference. Replacing our previous oncology panel (Proseek Multiplex Oncology I v2 96×96), this panel provides 60 completely new, high quality cancer-related biomarker assays, reducing overlap with other Proseek Multiplex panels and futher improving overall assay performance.

    An overview of how the 92 biomarker assays in the panel are classified according to Biological Process, Disease Area, Tissue Expression and Protein Class (based on widely used public-access bioinformatic databases, including Uniprot, Human Protein Atlas, Gene Ontology (GO) and DisGeNET) can be seen in the charts below. These charts are interactive: click on a chart-bar or pie-segment to see the list of biomarkers in that classification. Each item in the list links to the detailed biomarker page with validation data.

    Biological Process

    Disease Area

    Tissue Expression

    Protein Class

    For optimal assay performance, each immunoassay in the panel has been assessed in terms of specificity, precision, sensitivity, dynamic range, matrix effects and interference in biological material.

    A comprehensive list of all protein biomarkers that can be measured using the currently available Proseek Multiplex panels can be seen on the biomarker library page.

    Note: A printable list of the biomarker assays available in this panel, as well as a full data validation document, can be downloaded from our Document download center >>

    If you prefer not to run the assay yourself, let our experts here at Olink do this for you via our Analysis Service.

    The biomarker pages linked to below include calibrator curves that show the performance of each assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens, with data presented as Normalized Protein eXpression (NPX) values plotted against protein concentration (in pg/mL). Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please view the FAQ.

  • Search all our biomarkers

      Biomarkers in Proseek Multiplex Oncology II

      • 5'-nucleotidase (5'-NT)

        5'-nucleotidase, also known as CD73 (Cluster of Differentiation 73), is an enzyme that hydrolyzes extracellular nucleotides AMP, NAD & NMN) into membrane permeable nucleosides.D...

      • A disintegrin and metalloproteinase with thrombospondin motifs 15 (ADAM-TS 15)

        ADAM-TS 15 is a member of the ADAM (a disintegrin and metalloproteinase) family of type I transmembrane glycoproteins, which are involved in cell adhesion and proteolytic ectodo...

      • Alpha-taxilin (TXLNA)

        Alpha-taxilin (also known as interleukin-14) is a cytokine involved in regulating the proliferation of normal and cancerous B cells. As well as inducing B-cell proliferation, th...

      • Amphiregulin (AREG)

        Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

      • Annexin A1 (ANXA1)

        Annexin A1 belongs to a family of calcium-dependent phospholipid-binding proteins,and has roles in membrane fusion and exocytosis. It is principally located on the cytosolic fa...

      • C-type lectin domain family 4 member K (CD207)

        C-type lectin domain family 4 member K (CD207), also known as Langerin, is a type II transmembrane, C-type lectin receptor expressed in the immature dendritic cells of the epide...

      • C-X-C motif chemokine 13 (CXCL13 )

        C-X-C motif chemokine 13 (CXCL13) also known as B lymphocyte chemoattractant (BLC), is a small cytokine belonging to the CXC chemokine family. CXCL13 and its receptor CXCR5 cont...

      • Carbonic anhydrase IX (CAIX)

        CA9 is considered to be one of the best cellular biomarkers of hypoxia. Furthermore, recent studies examining the association between CA9 levels and various clinicopathological ...

      • Carboxypeptidase E (CPE)

        Carboxypeptidase E (also known as carboxypeptidase H, or convertase) is an exopeptidase that activates neuropeptides and peptide hormones, by cleaving off basic C-terminal amin...

      • Carcinoembryonic antigen (CEA)

        Carcinoembryonic antigen (CEA) is a glycoprotein involved in cell adhesion. CEA measurement is mainly used as a tumor marker to identify recurrences after surgical resection, or...

      • Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1)

        Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), also known as CD66a is a glycoprotein belonging to the immunoglobulin superfamily. CEACAM1 functions as a ce...

      • Cathepsin L2 (CTSV)

        Cathepsin L2 (also known as cathepsin V) is a lysosomal cysteine proteinase and a member of the peptidase C1 family. It is thought to play an important role in corneal physiolog...

      • CD160 antigen (CD160)

        CD160 antigen is a glycoprotein which functions as a receptor showing broad specificity for both classical and non-classical MHC class I molecules. It has also been investigated...

      • CD27 antigen (CD27)

        CD27 antigen is a member of the tumor necrosis factor receptor superfamily, and is needed for generation and maintenance of T cell immunity. Via its interactions with ligand CD7...

      • CD48 antigen (CD48)

        CD48 antigen, also known as B-lymphocyte activation marker (BLAST-1) or signaling lymphocytic activation molecule 2 (SLAMF2) is a member of the CD2 subfamily of the immunoglobul...

      • CD70 antigen (CD70)

        CD70 antigen is expressed on highly activated lymphocytes, where it functions as a ligand for CD27. Due to its preferential expression in lymphomas compared to normal lymphocyte...

      • Cornulin (CRNN)

        Cornulin, also known as 53 kDa squamous epithelial-induced stress protein or 58 kDa heat shock protein, is a putative calcium-binding protein that has been demonstrated to funct...

      • Cyclin-dependent kinase inhibitor 1 (CDKN1A)

        CDKN1A (also known as p21, WAF1) is a potent cyclin-dependent kinase inhibitor. The CDKN1A protein binds to and inhibits the activity of cyclin-CDK2, -CDK1, and -CDK4/6 complexe...

      • Delta-like protein 1 (DLL1)

        Delta-like protein 1, also known as Drosophila Delta homolog 1, acts as a ligand for Notch receptors, mediating cell fate decisions during hematopoiesis. Specifically, this prot...

      • Disintegrin and metalloproteinase domain-containing protein 8 (ADAM8)

        A Disintegrin and metalloproteinase domain-containing protein 8 (ADAM8), also known as cell surface antigen MS2, is a member of the ADAM family of membrane-anchored proteins. Th...

      • Endothelial cell-specific molecule 1 (ESM-1)

        ESM-1 (Endocan) has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Endocan expression has been shown to be increase in presence...

      • Ephrin type-A receptor 2 (EPHA2)

        Ephrin type-A receptor 2 (EPHA2), also known as Epithelial cell kinase, is a receptor tyrosine kinase which interacts with membrane-bound ephrin-A family ligands and triggers co...

      • Fas antigen ligand (FasL)

        Tumor necrosis factor ligand superfamily member 6 (FASLG), also known as Fas antigen ligand (FasL), induces apoptosis by binding to its receptor and forming the death-inducing s...

      • FAS-associated death domain protein (FADD)

        FADD, or MORT1, is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death...

      • Fc receptor-like B (FCRLB)

        Fc receptor-like B (FCRLB), also known as Fc receptor-like protein 2, belongs to the Fc receptor-like molecules (FCRLs). This is a family that resembles Fc receptors, with indiv...

      • Fibroblast growth factor-binding protein 1 (FGF-BP1)

        Fibroblast growth factor-binding protein 1 (FGFBP1) functions as a carrier protein that binds and releases fibroblast growth factors (FGFs) from the extracellular matrix, thereb...

      • Folate receptor alpha (FR-alpha)

        Folate receptor alpha can be overexpressed by a number of epithelial-derived tumors including ovarian, breast, renal, lung, colorectal, and brain. Hence antibodies to it are use...

      • Folate receptor gamma (FR-gamma)

        Folate receptor gamma (FR-gamma) is one of the isoforms produced by the folate receptor gene, FOLR3. FR-gamma binds to folate and its derivatives and mediates the delivery of 5-...

      • Furin (FUR)

        Furin is an enzyme which belongs to the subtilisin-like proprotein convertase family. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into ...

      • Galectin-1 (Gal-1)

        Galectin-1 (Gal-1) belongs to a family of proteins defined by their binding specificity for ?-galactoside sugars, but which unlike most membrane-bound lectins, are soluble prote...

      • Glypican-1 (GPC1)

        Glypican-1 (GPC1) is a cell surface heparan sulfate proteoglycan that binds alpha-4 (V) collagen and participates in Schwann cell myelination. The protein is required for normal...

      • Granzyme B (GZMB)

        Granzyme B (GZMB), also known as Granzyme-2 and human lymphocyte protein, is a serine protease that is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. ...

      • Granzyme H (GZMH)

        Granzyme H (GZMH), also known as cytotoxic serine protease C and cytotoxic T-lymphocyte proteinase, is a chymotrypsin-like serine protease with cytotoxic activity. The enzyme ha...

      • Hepatocyte growth factor (HGF)

        HGF is a growth, motility and morphogenic factor for a plethora of tissues and cell types and it plays important roles in angiogenesis, tumorogeneis, and tissue regeneration. HG...

      • ICOS ligand (ICOSLG)

        Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and ...

      • Insulin-like growth factor 1 receptor (IGF1R)

        Insulin-like growth factor 1 receptor (IGFR1) is a transmembrane receptor tyrosine kinase. Its main function is to mediate the actions of insulin-like growth factor 1 (IGF1), bu...

      • Integrin alpha-V (ITGAV)

        Integrin alpha-V (ITGAV), also known as CD antigen 51, undergoes post-translational cleavage to yield disulfide-linked heavy and light chains which in turn form heterodimers wit...

      • Integrin beta-5 (ITGB5)

        Integrin beta-5 (ITGB5) is a member of the integrin family of heterodimeric receptors, which consist of alpha and beta chains joined via disulphide bridges.Integrins act as rece...

      • Interferon gamma receptor 1 (IFN-gamma-R1)

        Interferon gamma receptor 1 (IFN gamma R1), also known as CD119, constitutes the ligand-binding alpha chain of the heterodimeric gamma interferon receptor. This receptor present...

      • Interleukin-6 (IL6)

        Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig...

      • Kallikrein-11 (hK11)

        Plasma kallikrein is expressed solely in liver and it is involved in blood clotting, fibrinolysis, regulation of blood pressure and inflammatory reactions.

      • Kallikrein-13 (KLK13)

        Kallikrein-13 (KLK13), also known as Kallikrein-like protein 4, is a member of the Kallikrein family of serine proteases. Kallikreins have diverse physiological functions, but t...

      • Kallikrein-14 (hK14)

        Kallikrein-14 (KLK14), also known as Kallikrein-like protein 6, is a member of the Kallikrein family of serine proteases. KLK14 exhibits endopeptidase activity with a dual tryps...

      • Kallikrein-8 (hK8)

        Kallikrein-8 (hK8), also known as Neuropsin and Ovasin, is a member of the Kallikrein family of serine proteases. The know targets for hK8 include casein, fibrinogen, kininogen,...

      • Ly6/PLAUR domain-containing protein 3 (LYPD3)

        Ly6/PLAUR domain-containing protein 3 (LYPD3), also known as GPI-anchored metastasis-associated protein C4, is a cell membrane-anchored glycoprotein. Expression is seen primaril...

      • Melanoma-derived growth regulatory protein (MIA)

        Melanoma-derived growth regulatory protein inhibits the growth of melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.It is a marker for mali...

      • Mesothelin (MSLN)

        Mesothelin (MSLN), also known as CAK1, is found attached to the cell membrane of normal mesothelial cells. Its precise functions are not yet known, but it is believed to play so...

      • Methionine aminopeptidase 2 (MetAP 2)

        Methionine aminopeptidase 2 (MetAP 2), also known as Initiation factor 2-associated 67 kDa glycoprotein, is a member of the dimetallohydrolase family. MetAP 2catalyzes the hydro...

      • MHC class I polypeptide-related sequence A/B (MIC-A/B)

        MHC class I polypeptide-related sequences A/B are expressed on the cell surface, although unlike canonical class I molecules do not seem to associate with beta-2-microglobulin. ...

      • Midkine (MK)

        Midkine (MK or MDK) also known as neurite growth-promoting factor 2 (NEGF2) is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophi...

      • Mothers against decapentaplegic homolog 5 (MAD homolog 5)

        Mothers against decapentaplegic homolog 5 (MADH5) also known as SMAD5, is named after its Drosophila homolog and is a member of the SMAD family of proteins, which are part of th...

      • Mucin-16 (MUC-16)

        Mucin 16, also known as ovarian cancer-related tumor marker 125 (CA-125), is a member of the mucin family of glycoproteins. It has found application as a tumor marker or biomark...

      • Nectin-4 (PVRL4)

        Nectin-4 (PVRL4), also known as Poliovirus receptor-related protein 4, is part of the Nectin family of calcium-independent cellular adhesion proteins.Nectins have adhesive roles...

      • Pancreatic prohormone (PPY)

        Pancreatic prohormone (PPY), also known as Pancreatic polypeptide, is produced in the pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal ...

      • Podocalyxin (PODXL)

        Podocalyxin (PODXL), also known as GCTM-2 antigen, is a member of the sialomucin protein family. It was first identified as a key component of the highly differentiated epitheli...

      • Pro-epidermal growth factor (EGF)

        EGF is prototypic of a family of growth factors that are derived from membrane-anchored precursors. A wide variety of in vitro and in vivo biological effects have been ascribed ...

      • Protein CYR61 (CYR61)

        Protein CYR61 (CYR61), also known as CCN family member 1, is a secreted, extracellular matrix (ECM)-associated signaling protein. CYR61 regulates a broad range of cellular activ...

      • Protein S100-A11 (S100A11)

        Protein S100-A11 (S100A11), also known as Calgizzarin and Metastatic lymph node gene 70 protein, is a member of the S100 family of proteins, which all contain 2 EF-hand calcium-...

      • Protein S100-A4 (S100A4)

        Protein S100-A4 (S100A4), also known as Calvasculin and Metastasin, is a member of the S100 family of proteins, which all contain 2 EF-hand calcium-binding motifs. S100 proteins...

      • Proto-oncogene tyrosine-protein kinase receptor Ret (RET)

        Proto-oncogene tyrosine-protein kinase receptor Ret (RET), also known as Cadherin family member 12, is a receptor tyrosine kinase for members of the glial cell line-derived neur...

      • R-spondin-3 (RSPO3)

        R-spondin-3 (RSPO3) is a member of the thrombospondin type 1 repeat supergene family and functions as an activator of the canonical Wnt signaling pathway by acting as a ligand f...

      • Receptor tyrosine-protein kinase erbB-2 (ErbB2/HER2)

        ERBB2 is a protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Defects in ERBB2 are or ma...

      • Receptor tyrosine-protein kinase erbB-3 (ErbB3/HER3)

        ErbB3, also called Her3 (human epidermal growth factor receptor 3) in humans, is a type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase recep...

      • Receptor tyrosine-protein kinase erbB-4 (ErbB4/HER4)

        Receptor tyrosine-protein kinase ErbB-4 is a member of the epidermal growth factor receptor subfamily. The protein binds to and is activated by neuregulins-2 and -3, heparin-bin...

      • Secretory carrier-associated membrane protein 3 (SCAMP3)

        Secretory carrier-associated membrane protein 3 (SCAMP3) is one of a family of secretory carrier membrane proteins. SCAMP 3 functions in post-Golgi recycling pathways, acting as...

      • Seizure 6-like protein (SEZ6L)

        Seizure 6-like protein (SEZ6L) is a transmembrane protein that may contribute to specialized endoplasmic reticulum functions in neurones.

      • SPARC (SPARC)

        SPARC (SPARC), also known as Osteonectin, is an acidic extracellular matrix glycoprotein that is secreted by osteoblasts during bone formation. It binds calcium and plays a vita...

      • Stem cell factor (SCF)

        SCF (c-Kit-ligand) is a cytokine that plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. SCF is up-regulated under inflammatory conditions and by stim...

      • Syndecan-1 (SYND1)

        Syndecan 1 (SYND1), also known as CD138, is a transmembrane (type I) heparan sulfate proteoglycan. It is a member of the syndecan family, which mediates cell binding, cell signa...

      • T-cell leukemia / lymphoma protein 1A (TCL1A)

        T-cell leukemia/lymphoma protein 1A (TCL1A), also known as Oncogene TCL-1, enhances the phosphorylation and activation of the AKT1, AKT2 and AKT3 kinases. TCL1A promotes nuclear...

      • T-lymphocyte surface antigen Ly-9 (LY9)

        T-lymphocyte surface antigen Ly-9 (LY9), also known as SLAMF3 and CD229, is a membrane protein that may participate in adhesion reactions between T lymphocytes and accessory cel...

      • TGF-beta receptor type-2 (TGFR-2)

        Transmembrane serine/threonine kinase that dimerizes with the TGF-beta type I receptor, forming the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. ...

      • Tissue factor pathway inhibitor 2 (TFPI-2)

        Tissue factor pathway inhibitor 2 (TFPI-2), also known as Placental protein 5, is a protease inhibitor that plays a role in the regulation of plasmin-mediated matrix remodeling....

      • TNF-related apoptosis-inducing ligand (TRAIL)

        TNF-related apoptosis-inducing ligand, TRAIL (also known as TNFSF10) is a cytokine that upon binding to its different receptors can activate apoptosis. However, its activity can...

      • Toll-like receptor 3 (TLR3)

        Toll-like receptor 3 (TLR3) also known as CD283, is a member of the Toll-like receptor family of pattern recognition receptors of the innate immune system. TLR3 is activated by ...

      • Transforming growth factor alpha (TGF-alpha)

        TGF-alpha is a growth factor produced in macrophages, brain cells, and keratinocytes, and upon its receptor EGFR it functions as an inducer of epithelial development. TGF-alpha ...

      • Transmembrane glycoprotein NMB (GPNMB)

        Transmembrane glycoprotein NMB (GPNMB) is expressed in a range of normal cell types such as melanocytes, osteoclasts, osteoblasts and dendritic cells, but more interestingly, is...

      • Tumor necrosis factor ligand superfamily member 13 (TNFSF13)

        Tumor necrosis factor ligand superfamily member 13 (TNFSF13), also known as A proliferation-inducing ligand (APRIL), is a member of the TNF ligand superfamily and is known to bi...

      • Tumor necrosis factor receptor superfamily member 19 (TNFRSF19)

        Tumor necrosis factor receptor superfamily member 19 (TNFRSF19), also known as TROY, interacts with TRAF family members and activates the JNK signaling pathway. The receptor is ...

      • Tumor necrosis factor receptor superfamily member 4 (TNFRSF4 )

        Receptor for TNFSF4/OX40L/GP34. Increasing evidence show that OX40 ligand (OX40L), also known as tumor necrosis factor superfamily member 4 (TNFSF4), plays an important role in ...

      • Tumor necrosis factor receptor superfamily member 6B (TNFRSF6B)

        Tumor necrosis factor receptor superfamily member 6B (TNFRSF6B), also known as Decoy receptor 3, is an important inhibitor of apoptosis. To achieve this, TNFRSF6B acts as a deco...

      • Tyrosine-protein kinase ABL1 (ABL1)

        Tyrosine-protein kinase ABL1 (ABL1), also known as Abelson murine leukemia viral oncogene homolog 1, plays a role in many key processes linked to cell growth and survival. These...

      • Tyrosine-protein kinase Lyn (LYN)

        Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, he...

      • Vascular endothelial growth factor A (VEGF-A)

        VEGF-A is a growth factor that upon binding to its receptors (VEGFR1 and VEGFR2) and to heparan sulfate and heparin, induces endothelial cell proliferation, promotes cell migrat...

      • Vascular endothelial growth factor receptor 2 (VEGFR-2)

        Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascula...

      • Vascular endothelial growth factor receptor 3 (VEGFR-3)

        Vascular endothelial growth factor receptor 3 (VEGFR-3) is a tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD. VEGFR-3 plays an essential role in...

      • VEGF-co regulated chemokine 1 (CXCL17)

        VEGF coregulated chemokine 1 (CXCL17), also known as Chemokine (C-X-C motif) ligand 17, is thought to be a housekeeping chemokine that regulates the recruitment of nonactivated ...

      • Vimentin (VIM)

        Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. VIM has been used as a sarcoma tumor marker to identify mesenchyme. Methylation...

      • WAP four-disulfide core domain protein 2 (WFDC2)

        WAP four-disulfide core domain protein 2 (WFDC2), also known as Human Epididymis Protein 4, is a broad range protease inhibitor. It is a member of the WFDC domain family and is ...

      • Wnt inhibitory factor 1 (WIF-1)

        Wnt inhibitory factor 1 (WIF-1) is a lipid-binding protein that binds to Wnt proteins and prevents them from triggering their normal signalling pathways. Wnt proteins are extrac...

      • WNT1-inducible-signaling pathway protein 1 (WISP-1)

        WNT1-inducible-signaling pathway protein 1 (WISP-1), also known as CCN4, is a member of the CCN family of secreted, extracellular matrix (ECM)-associated signaling proteins. WIS...

      • Xaa-Pro aminopeptidase 2 (XPNPEP2)

        Xaa-Pro aminopeptidase 2 (XPNPEP2), also known as Membrane-bound aminopeptidase P, is a metalloprotease that may play a role in the inflammatory process and other reactions prod...