This white paper describes the development of a customized 19-plex panel based on a new PEA protocol. With optimized molecular design and new conjugation chemistry, this enabled an assay in which high and low-abundant analytes could be combined into a single panel. In the proof-of-principle study described in the paper, calibrators were used for normalization and to enable absolute quantification of the measured proteins. Moreover, new validation processes are described which aim to ensure that panel is fit-for-purpose for future clinical utility.

Download the white paper here.