Assay Setup

• Why do the negative control and interplate control samples flag/not pass the quality control (QC)?
• How many replicates of each sample are analyzed?
• Should I dilute my samples before shipping them to Olink?
• Why would a sample not pass quality control in only one out of several panels run?
• I see that the same protein can be measured by more than one panel, are these the same assay?
• How is it determined whether a sample has failed quality control because of operator error or due to some property of the sample?
• Why do some of your biomarkers have several Uniprot numbers?
• Is it possible to run formalin fixed samples with Olink?
• What is the maximum sample throughput?
• Why is the limit for a sample passing or failing the quality control set to +/- 0.3NPX of the plate median for Incubation control 2 and the detection control?