Olink Explore contains three types of internal controls spiked into our assay that monitor every step of the procedure.
Incubation Control is PEA assay for non-human proteins which monitor potential variation across all steps, and used in data QC.
Extension Control is an antibody molecule with both DNA-oligos attached and therefore always in proximity. This controls for the extension, library preparation and sequencing steps and is also used for data normalization purposes.
Amplification Control is a synthetic double-stranded amplicon which controls the library preparation and sequencing step of the protocol and used in data QC.