IL-2 is a cytokine that upon binding to its receptor IL-2R stimulates proliferation of T cells, but it can also stimulate other immune cells. IL-2 plays and important role in T cell immunologic memory as well as in maturation of regulatory T cells for regulation of autoimmunity. IL-2 was the first interleukin molecule to be identified and characterized, and it has been described in many inflammation-related diseases. IL-2 has a clinical application in the treatment of certain cancers and is being tested as an adjuvant in vaccines in the treatment of chronic viral infections. Note: This assay does not detect the low levels of IL-2 present in plasma from healthy individuals. The assay does, however, detect IL-2 in plasma, serum and other sample types from inflammatory diseases where higher levels of this protein are expressed.
Recommended sample types are EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact email@example.com for more information.
Analytical Measuring Range
Please note: the technical data reported below refers to the measured value in the in vitro validation assays. The calibrator curves are generated during the assay validation process using recombinant antigens, with data presented as Normalized Protein eXpression (NPX) values plotted against protein concentration (in pg/mL). Please note that when analyzing biological samples the in vitro-generated curves presented here cannot be used to derive protein concentrations in pg/mL from the NPX values obtained. Olink Target 48 Cytokine delivers absolute protein concentrations directly using the calibrator system built into the system. For more information about NPX measurements, please view the FAQ.
Calibrator curve for validation data (generated in multiplex)
Within run precision Coefficient of Variation (CV)
Between run precision Coefficient of Variation (CV)
Precision (repeatability) is calculated on quantified data within the limit of quantification: LOQ, between lower limit of quantification (LLOQ) and upper limit of quantification (ULOQ).
Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents – go to Document download center