Granulocyte colony-stimulating factor, G-CSF (also known as pluripoietin) is a glycoprotein hormone that functions as a cytokine and induces granulocytes via stimulation of the bone marrow. In addition to this general role in hematopoiesis, G-CSF also functions as a neurotrophic factor, with multiple effects on neurons mediated via a receptor that is expressed in neurons in the brain and spinal cord. In this capacity, G-CSF is involved in inducing neurogenesis, enhancing neuroplasticity and inhibiting neronal apoptosis. Due these properties, G-CSF has been the subject of investigations to assess its potential as a treatments for neurological diseases such as cerebral ischemia. The protein has also been implicated in stroke.
Recommended sample types are EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact firstname.lastname@example.org for more information.
Analytical Measuring Range
Please note: the technical data reported below refers to the measured value in the in vitro validation assays. The calibrator curves are generated during the assay validation process using recombinant antigens, with data presented as Normalized Protein eXpression (NPX) values plotted against protein concentration (in pg/mL). Please note that when analyzing biological samples the in vitro-generated curves presented here cannot be used to derive protein concentrations in pg/mL from the NPX values obtained. Olink Target 48 Cytokine delivers absolute protein concentrations directly using the calibrator system built into the system. For more information about NPX measurements, please view the FAQ.
Calibrator curve for validation data (generated in multiplex)
Within run precision Coefficient of Variation (CV)
Between run precision Coefficient of Variation (CV)
Precision (repeatability) is calculated on quantified data within the limit of quantification: LOQ, between lower limit of quantification (LLOQ) and upper limit of quantification (ULOQ).
Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents – go to Document download center