Granzyme B (GZMB), also known as Granzyme-2 and human lymphocyte protein, is a serine protease that is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Consequently, GZMB has a crucial role in the induction of infected target cell apoptosis as part of the cell-mediated immune response. This mechanism is believed to involve cleavage of caspase-3, -7, -9 and 10, giving rise to the active enzymes that mediate apoptosis.
Recommended sample types are EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact firstname.lastname@example.org for more information.
Analytical Measuring Range
Please note: the technical data reported below refers to the measured value in the in vitro validation assays. The calibrator curve below shows the performance of the assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please visit our FAQ page (https://www.olink.com/question/what-is-npx).
Calibrator curve generated in multiplex
Within run precision Coefficient of Variation (CV)
Between run precision Coefficient of Variation (CV)
Precision (repeatability) is calculated from linearized NPX values over LOD.
Biomarker Validation Data
Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents – go to Document download center