• Ubiquitin carboxyl-terminal hydrolase 8 (USP8)

    Links to databases

    Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1.

    Sample type

    Recommended sample types are EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.

    Analytical Measuring Range

    Please note: the technical data reported below refers to the measured value in the in vitro validation assays. The calibrator curve below shows the performance of the assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please visit our FAQ page (https://www.olink.com/question/what-is-npx).

    Since the Metabolism panel uses human samples diluted 10-fold, a multiplication factor of 10 should be applied when comparing the addressable biological concentration to this validation data.

    LOD (pg/mL)
    244
    LLOQ (pg/mL)
    488
    ULOQ (pg/mL)
    250000
    Hook (pg/mL)
    1000000
    Range (logs)
    2.7

    Calibrator curve for validation data (generated in multiplex)

    Ubiquitin carboxyl-terminal hydrolase 8 (USP8)

    Precision

    Within run precision Coefficient of Variation (CV)
    9%
    Between run precision Coefficient of Variation (CV)
    12%

    Precision (repeatability) is calculated from linearized NPX values over LOD.

    Biomarker Validation Data

    Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents – go to Document download center

    2947

    Biomarker assays

    ~881 million

    Protein data points generated

    886

    Publications listed on website

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