Neurogenic locus notch homolog protein 3 (Notch 3)

Links to databases

 

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Mutations in the NOTCH3 gene have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). Mutations in NOTCH3 are also associated to the rare lateral meningocele syndrome. Furthermore, mutations in NOTCH3 have also been identified in a Turkish family with Alzheimer's disease.

Sample type

Recommended sample types are human EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.

NOTE: The calibrator curve below shows the performance of the assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please visit our FAQ page.

Analytical Measuring Range

Please note: the technical data reported below refers to the measured value in the in vitro validation assays. Since the CVD III panel uses human samples diluted 100-fold, a multiplication factor of 100 should be applied when comparing the addressable biological concentration to this validation data.

    • LOD (pg/mL)
    • 1.91
    • LLOQ (pg/mL)
    • 3.81
    • ULOQ (pg/mL)
    • 62500
    • Hook (pg/mL)
    • 62500
    • Range (logs)
    • 4.2

Calibrator curve for validation data (generated in multiplex)

Neurogenic locus notch homolog protein 3 (Notch 3)

Precision

    • Within run precision Coefficient of Variation (CV)
    • 9%
    • Between run precision Coefficient of Variation (CV)
    • 10%

Precision (repeatability) is calculated from linearized NPX values over LOD.

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents. To download or to learn more go to the Data Validation page.

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