Interleukin-33 (IL-33)

Links to databases

 

IL-33 has been described as both a pro-inflammatory and immunoregulatory cytokine that can bind two different receptors: IL1RL1 (ST2) and IL1RAP. IL-33 induces production of other cytokines e.g. IFN-gamma, acts as a chemoattractant for T cells, as well as signal tissue and cell damage in order to amplify immune responses. IL-33 has been described to be involved in several inflammation-related diseases such as allergy induced airway inflammation, atherosclerosis, etc. and it has been suggested as an adjuvant in vaccines against pathogens. Note: This assay does not detect the low levels of IL-33 present in plasma from healthy individuals. IL-33 can be detected, however, in plasma, serum and other sample types from inflammatory diseases where higher levels of this protein are expressed.

Sample type

Recommended sample types are human EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.

NOTE: The calibrator curve below shows the performance of the assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please visit our FAQ page.

Analytical Measuring Range

    • LOD (pg/mL)
    • 3.81
    • LLOQ (pg/mL)
    • 3.81
    • ULOQ (pg/mL)
    • 31250
    • Hook (pg/mL)
    • 125000
    • Range (logs)
    • 3.9

Calibrator curve generated in multiplex

Interleukin-33 (IL-33)

Precision

    • Within run precision Coefficient of Variation (CV)
    • 9%
    • Between run precision Coefficient of Variation (CV)
    • 26%

Precision (repeatability) is calculated from linearized NPX values over LOD.

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents. To download or to learn more go to the Data Validation page.

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