Interferon gamma (IFN-gamma)

Links to databases

 

IFN-gamma is a cytokine that plays critical roles in innate and adaptive immunity against viral and bacterial infections as well as tumor control. It functions as a stimulator and modulator of the immune response and it has been studied in many inflammation-related diseases including autoinflammatory and autoimmune conditions. IFN-gamma has therapeutic uses for the treatment of chronic granulomatous disease and osteopetrosis. Note: This assay cannot usually detect the low levels of IFN gamma present in plasma from healthy individuals. The assay does, however, detect IFN-gamma in plasma and serum samples from several inflammatory diseases where higher levels of this protein are expressed. One example is the study by Brunner et al. where increases of IFN-gamma were detected in patients with atopic dermatitis and psoriasis compared to controls. Brunner et al. The atopic dermatitis blood signature is characterized by increases in inflammatory and cardiovascular risk proteins. Sci Rep. 2017; 7: 8707.

Sample type

Recommended sample types are human EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.

NOTE: The calibrator curve below shows the performance of the assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please visit our FAQ page.

Analytical Measuring Range

    • LOD (pg/mL)
    • 15.26
    • LLOQ (pg/mL)
    • 15.26
    • ULOQ (pg/mL)
    • 62500
    • Hook (pg/mL)
    • 62500
    • Range (logs)
    • 3.6

Calibrator curve generated in multiplex

Interferon gamma  (IFN-gamma)

Precision

    • Within run precision Coefficient of Variation (CV)
    • 7%
    • Between run precision Coefficient of Variation (CV)
    • 17%

Precision (repeatability) is calculated from linearized NPX values over LOD.

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents. To download or to learn more go to the Data Validation page.

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