The most comprehensive multiplexed protein biomarker panel designed for mouse studies
Using the same, well-established technology and high standards behind Olink’s portfolio of high-multiplex panels for human protein biomarker discovery, Olink MOUSE EXPLORATORY offers 92 high-quality assays for murine proteins. The proteins assayed in the panel are all known to be detectable in human serum and plasma, optimizing the human relevance of any mouse study findings. The 92 proteins in the panel also encompass a broad range of biological functions and pathways, making this an ideal tool for exploratory studies aimed at discovering new biomarkers and protein signatures in mouse studies.
Categorization of the proteins included in the panel was carried out via referral to widely used public-access bioinformatic databases, including Uniprot and Gene Ontology (GO). The assays in this panel include proteins involved in key biological processes such as cellular regulation, development, signal transduction and stress responses.
The mouse panel includes:
- Assays from our current human panels – for proteins with high human/murine homology, where the existing assay shows good technical functionality with mouse samples
- New assays developed specifically for mouse samples and this panel.
The Mouse Exploratory panel offers simultaneous analysis of 92 protein biomarkers, consuming just 1 µL of biological sample, and all assays are subject to extensive technical validation and QC control procedures. The selection of protein biomarker assays is designed to provide the optimal dynamic range, as well as to provide proteins that cover a broad range of important biology.
An alphabetical list of the 92 proteins with links to the individual biomarker pages containing additional information and assay validation data can be found at the bottom of this page.
Pilot study application examples
Some early examples of how the panel can be used in a broad range of applications were generated during customer pilot studies carried out during the product development process.
Read more about these in the document, Olink Mouse Exploratory – Pilot Studies
Classification of proteins in the panel
An overview of how the 92 biomarker assays in the panel are classified according to Biological Process and Intracellular Location can be seen in the charts below. These charts are interactive: click on a chart-bar or pie-segment to see the list of biomarkers in that classification. Each item in the list links to the detailed biomarker page with validation data. The number of proteins from the panel in each category are shown in the bar and pie charts below. Note that proteins can fall into multiple categories. Biological processes were defined by the GO terms corresponding to the mouse Uniprot IDs; subcellular locations were as defined on the Uniprot pages for each mouse protein.
Note: A printable list of the biomarker assays available in this panel, as well as a full data validation document, can be downloaded from our Document download center >>
If you prefer not to run the assay yourself, let our experts here at Olink do this for you via our Analysis Service.
Some of the biomarker pages linked to below include calibrator curves that show the performance of each assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens, with data presented as Normalized Protein eXpression (NPX) values plotted against protein concentration (in pg/mL). Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please view the FAQ. Note that recombinant mouse antigens were only available for around half of the assays in this panel, so that the remainder do not have calibrator curves available.