Tyrosine-protein kinase Yes - mouse (Yes1)

Links to databases

Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis (By similarity).

Sample type

Recommended sample types are human EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.

NOTE: The calibrator curve below shows the performance of the assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please visit our FAQ page.

Analytical Measuring Range

    • LOD (pg/mL)
    • NA
    • LLOQ (pg/mL)
    • NA
    • ULOQ (pg/mL)
    • NA
    • Hook (pg/mL)
    • NA
    • Range (logs)
    • NA

Calibrator curve for validation data (generated in multiplex)

Tyrosine-protein kinase Yes - mouse (Yes1)

Detectability in different mouse sample matrices

Tyrosine-protein kinase Yes - mouse (Yes1)

The figure above shows the overall detectability of the specified assay in a range of different mouse sample types. Detectability in each sample matrix is presented as the mean % of all samples included in the validation studies that could be measured above the limit of detection (LOD).

Dilution of samples may be necessary for some sample matrices other than plasma or serum to achieve optimal assay performance – the data shown above represents the values obtained at the optimal dilution identified in each case. Details regarding possible dilutions should be discussed with your Olink representative prior to commencing the study. The data is compiled from multiple studies comprising a range of healthy and disease samples, including samples from genetically modified mouse lines in some cases.

(Note: CSF = cerebrospinal fluid; BAL = bronchoalveolar lavage).

Precision

    • Within run precision Coefficient of Variation (CV)
    • 5%
    • Between run precision Coefficient of Variation (CV)
    • 12%

Precision (repeatability) is calculated from linearized NPX values over LOD.

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents. To download or to learn more go to the Data Validation page.

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