Melanoma-derived growth regulatory protein - mouse (Mia)

Links to databases

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

Sample type

Recommended sample types are human EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.

NOTE: The calibrator curve below shows the performance of the assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please visit our FAQ page.

Analytical Measuring Range

    • LOD (pg/mL)
    • NA
    • LLOQ (pg/mL)
    • NA
    • ULOQ (pg/mL)
    • NA
    • Hook (pg/mL)
    • NA
    • Range (logs)
    • NA

Calibrator curve for validation data (generated in multiplex)

Melanoma-derived growth regulatory protein - mouse (Mia)

Detectability in different mouse sample matrices

Melanoma-derived growth regulatory protein - mouse (Mia)

The figure above shows the overall detectability of the specified assay in a range of different mouse sample types. Detectability in each sample matrix is presented as the mean % of all samples included in the validation studies that could be measured above the limit of detection (LOD).

Dilution of samples may be necessary for some sample matrices other than plasma or serum to achieve optimal assay performance – the data shown above represents the values obtained at the optimal dilution identified in each case. Details regarding possible dilutions should be discussed with your Olink representative prior to commencing the study. The data is compiled from multiple studies comprising a range of healthy and disease samples, including samples from genetically modified mouse lines in some cases.

(Note: CSF = cerebrospinal fluid; BAL = bronchoalveolar lavage).

Precision

    • Within run precision Coefficient of Variation (CV)
    • 5%
    • Between run precision Coefficient of Variation (CV)
    • 14%

Precision (repeatability) is calculated from linearized NPX values over LOD.

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents. To download or to learn more go to the Data Validation page.

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