Transmembrane glycoprotein NMB (GPNMB)

Links to databases

Transmembrane glycoprotein NMB (GPNMB) is expressed in a range of normal cell types such as melanocytes, osteoclasts, osteoblasts and dendritic cells, but more interestingly, is overexpressed in many types of cancer. While initial observations linked GPNMB specifically with poorly metastatic human melanoma cells, wider studies have also shown its expression in highly aggressive melanoma, glioma and breast cancer specimens. GPNMB is also thought to have potential as a prognostic marker for breast cancer recurrence and reduced metastasis-free survival times. At least one drug targeting GPNMB for cancer therapy applications has been taken to clinical trials.

Sample type

Recommended sample types are human EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.

NOTE: The calibrator curve below shows the performance of the assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please visit our FAQ page.

Analytical Measuring Range

    • LOD (pg/mL)
    • 30.5
    • LLOQ (pg/mL)
    • 61
    • ULOQ (pg/mL)
    • 62500
    • Hook (pg/mL)
    • 125000
    • Range (logs)
    • 3

Calibrator curve for validation data (generated in multiplex)

Transmembrane glycoprotein NMB (GPNMB)

Precision

    • Within run precision Coefficient of Variation (CV)
    • 4%
    • Between run precision Coefficient of Variation (CV)
    • 20%

Precision (repeatability) is calculated from linearized NPX values over LOD.

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents. To download or to learn more go to the Data Validation page.

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