Sialoadhesin (SIGLEC1)

Links to databases

Sialoadhesin, SIGLEC1 (also known as CD169) is a lectin found on the surface of macrophages that predominantly binds neutrophils, but also other immune system cell-types, by interacting with sialic acid molecules within their surface ligands. Sialoadhesin is expressed by macrophages in various tissues, with highest levels seen in spleen, lymph node and perivascular macrophages in the brain. Sialaodhesin-positive macrophages, along with mesenchymal stem cells and beta-adrenergic neurons, form the hematopoietic stem cell niche in the bone marrow. These macrophages mediate signaling between the various cells and seem to promote hematopoietic stem cell retention to the niche. There is intriguing evidence from animal experiments that the murine homolog of Sialoadhesin may play an agonistic role in demyelination in some inherited neuropathies.

Sample type

Recommended sample types are human EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.

NOTE: The calibrator curve below shows the performance of the assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please visit our FAQ page.

Analytical Measuring Range

    • LOD (pg/mL)
    • 61.04
    • LLOQ (pg/mL)
    • 122.07
    • ULOQ (pg/mL)
    • 125000
    • Hook (pg/mL)
    • 250000
    • Range (logs)
    • 3

Calibrator curve generated in multiplex

Sialoadhesin (SIGLEC1)

Precision

    • Within run precision Coefficient of Variation (CV)
    • 6%
    • Between run precision Coefficient of Variation (CV)
    • 9%

Precision (repeatability) is calculated from linearized NPX values over LOD.

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents. To download or to learn more go to the Data Validation page.

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