N-terminal prohormone of brain natriuretic peptide
Uniprot Function Description
The N-terminal of the prohormone brain natriuretic peptide (NT-proBNP), also commonly abbreviated BNPT, is a 76 amino acid N-terminal inactive protein that is cleaved from proBNP to release brain natriuretic peptide. Both BNP and NT-proBNP levels in the blood are used for screening, diagnosis of acute congestive heart failure (CHF) and may be useful to establish prognosis in heart failure, as both markers are typically higher in patients with worse outcome. The plasma concentrations of both forms typically increase in patients with asymptomatic/symptomatic left ventricular dysfunction, which is associated with coronary artery disease and myocardial ischemia
NT-proBNP is cleavage product and consequently has no UniProt ID. The original full-length protein, Natriuretic peptides B, has UniProt ID P16860.
Precision (repeatability) is calculated from linearized NPX values over LOD.
Within run precision Coefficient of Variation (CV)
Between run precision Coefficient of Variation (CV)
Analytical measuring range
The technical data reported below refers to the measured value in the in vitro validation assays run using known concentrations of recombinant antigen. Please note that these figures are for reference only and CANNOT be used to convert NPX values to absolute concentrations for proteins measured in plasma or serum samples.
For optimal assay readout, Olink Explore is run using different dilutions of the original samples (undiluted, 1:10, 1:100 or 1:1000). The dilution factor for this assay is noted below and should be taken into account when estimating the appropriate addressable biological concentration of the protein based on the in vitro validation data.
The calibrator curve shown below visualizes the analytical measuring range data based on in vitro measurement of recombinant antigen. Please note that this is shown for reference only and CANNOT be used to convert NPX values to absolute concentrations for proteins measured in plasma or serum samples. The vertical dotted lines represent LLOQ and ULOQ respectively, and the horizontal line indicates the LOD. The y-axis shows NPX above background, which is defined as the median of negative control measurements and used to define the expected background level (zero).
Sample distribution plot
The plot below shows the levels of protein measured in a number of commercial plasma samples. Healthy subjects are shown in blue and samples obtained from patients with a range of diseases are shown in red. The latter include inflammatory, cardiovascular, autoimmune & neurological diseases, as well as cancer. The data is shown to give a general idea of the sort of NPX range to expect, but cannot cover all potential levels that may be seen in clinical samples. The y-axis shows NPX above background, which is defined as the median of negative control measurements and used to define the expected background level (zero).
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