Interferon gamma (IFN-gamma)
Links to databases
IFN-gamma is a cytokine that plays critical roles in innate and adaptive immunity against viral and bacterial infections as well as tumor control. It functions as a stimulator and modulator of the immune response and it has been studied in many inflammation-related diseases including autoinflammatory and autoimmune conditions. IFN-gamma has therapeutic uses for the treatment of chronic granulomatous disease and osteopetrosis. Note: This assay cannot usually detect the low levels of IFN gamma present in plasma from healthy individuals. The assay does, however, detect IFN-gamma in plasma and serum samples from several inflammatory diseases where higher levels of this protein are expressed. One example is the study by Brunner et al. where increases of IFN-gamma were detected in patients with atopic dermatitis and psoriasis compared to controls.
Brunner et al. The atopic dermatitis blood signature is characterized by increases in inflammatory and cardiovascular risk proteins. Sci Rep. 2017; 7: 8707.
Recommended sample types are EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact firstname.lastname@example.org for more information.
Analytical Measuring Range
Please note: the technical data reported below refers to the measured value in the in vitro validation assays. The calibrator curves are generated during the assay validation process using recombinant antigens, with data presented as Normalized Protein eXpression (NPX) values plotted against protein concentration (in pg/mL). Please note that when analyzing biological samples the in vitro-generated curves presented here cannot be used to derive protein concentrations in pg/mL from the NPX values obtained. Olink Target 48 Cytokine delivers absolute protein concentrations directly using the calibrator system built into the system. For more information about NPX measurements, please view the FAQ.
Calibrator curve for validation data (generated in multiplex)
- Within run precision Coefficient of Variation (CV)
- Between run precision Coefficient of Variation (CV)
Precision (repeatability) is calculated on quantified data within the limit of quantification: LOQ, between lower limit of quantification (LLOQ) and upper limit of quantification (ULOQ).