Tumor necrosis factor (TNF)

Links to databases

TNF (TNF-alpha) is a cytokine that upon binding to its receptors TNFRSF1A and TNFRSF1B activates NF-kappaB, MAPK, but also in a lower level apoptosis signaling pathways. TNF plays important roles in systemic inflammation and acute phase reaction and it has been described to be involved in several inflammation-related diseases such as fever, psoriatic arthritis, inflammatory bowel disease, rheumatoid arthritis, etc. Note: This assay does not detect the low levels of TNFa present in plasma from healthy individuals. The assay does, however, detect TNF in serum and plasma samples from several inflammatory diseases where higher levels of this proteins are expressed. One example is the study by Brunner et al. where TNF is measured both in patients with atopic dermatitis and psoriasis. Brunner et al. The atopic dermatitis blood signature is characterized by increases in inflammatory and cardiovascular risk proteins. Sci Rep. 2017; 7: 8707.

Sample type

Recommended sample types are EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.

Analytical Measuring Range

Please note: the technical data reported below refers to the measured value in the in vitro validation assays. The calibrator curves are generated during the assay validation process using recombinant antigens, with data presented as Normalized Protein eXpression (NPX) values plotted against protein concentration (in pg/mL). Please note that when analyzing biological samples the in vitro-generated curves presented here cannot be used to derive protein concentrations in pg/mL from the NPX values obtained. Olink Target 48 Cytokine delivers absolute protein concentrations directly using the calibrator system built into the system. For more information about NPX measurements, please view the FAQ.

    • LOD (pg/mL)
    • 1.907
    • LLOQ (pg/mL)
    • 3.81
    • ULOQ (pg/mL)
    • 15625
    • Hook (pg/mL)
    • 62500
    • Range (logs)
    • 3.6

Calibrator curve for validation data (generated in multiplex)

Tumor necrosis factor (TNF)

Precision

    • Within run precision Coefficient of Variation (CV)
    • 11%
    • Between run precision Coefficient of Variation (CV)
    • 13%

Precision (repeatability) is calculated on quantified data within the limit of quantification: LOQ, between lower limit of quantification (LLOQ) and upper limit of quantification (ULOQ).

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