Podocalyxin (PODXL)

Links to databases

 

Podocalyxin (PODXL), also known as GCTM-2 antigen, is a member of the sialomucin protein family. It was first identified as a key component of the highly differentiated epithelial cell-type, glomerular podocytes.PODXL has regulatory functions in both cell adhesion and morphology, via diverse mechanisms involving both pro- and anti-adhesive activities.Pro-adhesive functions appear to be integrin-dependent, whereas as it's anti-adhesive effects may be mediated by charge repulsion. Biological processes that depend on PODXL include renal tubulogenesis, formation of apical actin-dependent microvilli and hematopoetic cell differentiation. Clincally, PODXL is involved in cancer development by inducing cell migration and invasion through its interaction with the actin-binding protein EZR.

Sample type

Recommended sample types are human EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.

NOTE: The calibrator curve below shows the performance of the assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please visit our FAQ page.

Analytical Measuring Range

    • LOD (pg/mL)
    • 7.63
    • LLOQ (pg/mL)
    • 7.63
    • ULOQ (pg/mL)
    • 31250
    • Hook (pg/mL)
    • 125000
    • Range (logs)
    • 3.6

Calibrator curve generated in multiplex

Podocalyxin (PODXL)

Precision

    • Within run precision Coefficient of Variation (CV)
    • 5%
    • Between run precision Coefficient of Variation (CV)
    • 16%

Precision (repeatability) is calculated from linearized NPX values over LOD.

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents. To download or to learn more go to the Data Validation page.

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