SPARC (SPARC)

Links to databases

 

SPARC (SPARC), also known as Osteonectin, is an acidic extracellular matrix glycoprotein that is secreted by osteoblasts during bone formation. It binds calcium and plays a vital role in bone mineralization, cell-matrix interactions, and collagen binding. There are reported correlations between SPARC over-expression with both ampullary cancers and chronic pancreatitis. SPARC also increases the activity of matrix metalloproteinases, which are important for invading cancer cells within bone. SPARC may also contribute to other cancer-related functions, such as tumor cell proliferation and migration, as well as angiogenesis. Overexpression of SPARC has been reported in many human cancers such as breast, prostate and colon.

Sample type

Recommended sample types are human EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.

NOTE: The calibrator curve below shows the performance of the assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please visit our FAQ page.

Analytical Measuring Range

    • LOD (pg/mL)
    • 30.52
    • LLOQ (pg/mL)
    • 61.04
    • ULOQ (pg/mL)
    • 125000
    • Hook (pg/mL)
    • 1000000
    • Range (logs)
    • 3.3

Calibrator curve generated in multiplex

SPARC (SPARC)

Precision

    • Within run precision Coefficient of Variation (CV)
    • 5%
    • Between run precision Coefficient of Variation (CV)
    • 11%

Precision (repeatability) is calculated from linearized NPX values over LOD.

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents. To download or to learn more go to the Data Validation page.

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