R-spondin-3 (RSPO3)

Links to databases

 

R-spondin-3 (RSPO3) is a member of the thrombospondin type 1 repeat supergene family and functions as an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. This pathway causes cytoplasmic accumulation of beta-catenin, followed by translocation into the nucleus, where it functions as a coactivator for TCF/LEF family transcription. This pathway has multiple key functions in embryogenesis and cellular regulation post-development (e.g. cellular proliferation, differentiation and migration. Clinically, the importance of this pathway has been demonstrated in breast and prostate cancer, glioblastoma, type II diabetes, and many other diseases.

Sample type

Recommended sample types are human EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.

NOTE: The calibrator curve below shows the performance of the assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please visit our FAQ page.

Analytical Measuring Range

    • LOD (pg/mL)
    • 1.91
    • LLOQ (pg/mL)
    • 1.91
    • ULOQ (pg/mL)
    • 15625
    • Hook (pg/mL)
    • 31250
    • Range (logs)
    • 3.9

Calibrator curve generated in multiplex

R-spondin-3 (RSPO3)

Precision

    • Within run precision Coefficient of Variation (CV)
    • 10%
    • Between run precision Coefficient of Variation (CV)
    • 13%

Precision (repeatability) is calculated from linearized NPX values over LOD.

Biomarker Validation Data

Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents. To download or to learn more go to the Data Validation page.

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