Interleukin-6 - mouse (Il6)
Links to databases
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.
Recommended sample types are EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact firstname.lastname@example.org for more information.
Analytical Measuring Range
Calibrator curve for validation data (generated in multiplex)
Detectability in different mouse sample matrices
The figure above shows the overall detectability of the specified assay in a range of different mouse sample types. Detectability in each sample matrix is presented as the mean % of all samples included in the validation studies that could be measured above the limit of detection (LOD).
Dilution of samples may be necessary for some sample matrices other than plasma or serum to achieve optimal assay performance – the data shown above represents the values obtained at the optimal dilution identified in each case. Details regarding possible dilutions should be discussed with your Olink representative prior to commencing the study. The data is compiled from multiple studies comprising a range of healthy and disease samples, including samples from genetically modified mouse lines in some cases.
(Note: CSF = cerebrospinal fluid; BAL = bronchoalveolar lavage).
- Within run precision Coefficient of Variation (CV)
- Between run precision Coefficient of Variation (CV)
Precision (repeatability) is calculated from linearized NPX values over LOD.
Biomarker Validation Data
Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents. To download or to learn more go to the Data Validation page.