Repulsive guidance molecule A - mouse (Rgma)
Links to databases
Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.
Recommended sample types are EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact email@example.com for more information.
Analytical Measuring Range
Calibrator curve for validation data (generated in multiplex)
Detectability in different mouse sample matrices
The figure above shows the overall detectability of the specified assay in a range of different mouse sample types. Detectability in each sample matrix is presented as the mean % of all samples included in the validation studies that could be measured above the limit of detection (LOD).
Dilution of samples may be necessary for some sample matrices other than plasma or serum to achieve optimal assay performance – the data shown above represents the values obtained at the optimal dilution identified in each case. Details regarding possible dilutions should be discussed with your Olink representative prior to commencing the study. The data is compiled from multiple studies comprising a range of healthy and disease samples, including samples from genetically modified mouse lines in some cases.
(Note: CSF = cerebrospinal fluid; BAL = bronchoalveolar lavage).
- Within run precision Coefficient of Variation (CV)
- Between run precision Coefficient of Variation (CV)
Precision (repeatability) is calculated from linearized NPX values over LOD.
Biomarker Validation Data
Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents. To download or to learn more go to the Data Validation page.