Are internal controls included in the assay, and if so what are they?
The assay uses four internal controls that are added into all 96 wells of the plate. These are used for quality control and for data normalization. The controls monitor all the different steps of the Proximity Extension Assay (PEA): two of the controls monitor the immuno reaction (Incubation control 1 and Incubation control 2) and two of the controls monitor the extension and qPCR steps (Extension control and Detection control). The controls and how they are used is outlined below:
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