The Negative Control for Olink Explore consists of buffer run as a normal sample. These are used to monitor any background noise generated when DNA-tags come in close proximity without prior binding to the appropriate protein. In Explore 384/3072, the Negative Controls set the background levels for each protein assay and are used to calculate the limit of detection (LOD). In Explore HT, the Negative Controls are used assess potential contamination.
The Negative Control is run in duplicates on Explore HT and triplicates on Explore 384/3072.