Olink® Explore 3072

A high-throughput platform offering uncompromised data quality

Olink® Explore 3072 is a high-throughput protein biomarker discovery platform used to measure the concentration of thousands of human plasma proteins using minute amounts of sample. Explore 3072 comprises our entire library of validated protein assays.

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Key features of Olink Explore 3072

  • Comprises our entire protein library
  • Covers all major biological pathways
  • Extends the dynamic range of previous assays to cover higher abundance proteins
  • Significantly increases both sample and data throughput for each run
    ~4600 samples processed per week, per NGS instrument
    ~14 million protein data points generated per week, per NGS instrument
  • Maintains Olink’s unparalleled sensitivity, specificity, and precision at high multiplex

The Explore 3072 library consists of eight Explore 384-plex panels:

Olink® Explore 384 Cardiometabolic
Olink® Explore 384 Cardiometabolic II
Olink® Explore 384 Inflammation
Olink® Explore 384 Inflammation II
Olink® Explore 384 Neurology
Olink® Explore 384 Neurology II
Olink® Explore 384 Oncology
Olink® Explore 384 Oncology II
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Watch the video for more information on how Olink Explore 3072 enables the addition of game-changing proteomics to the multiomic approaches that are increasingly being used to address important questions in biomedical research.

Director of Human Genetics at Pfizer, Dr. Anders Mälarstig, gives his views on how this expanded offering from Olink will fill an important role in enabling large-scale, high quality proteomics studies that will give actionable biological insights across a broad range of human diseases.

What is Olink Explore?

The Olink Explore is a high-multiplex, high-throughput protein biomarker platform that uses Proximity Extension Assay (PEA) technology coupled combined with an innovative new readout methodology based on Next Generation Sequencing (NGS). The following Illumina® instruments are compatible with the Explore platform:

  • NovaSeq 6000
  • NextSeq 550
  • NextSeq 2000

The first version of the Explore platform, Explore 1536, was released in June 2020 and consisted of four 384-plex kits. Olink Explore 3072 offers an additional four 384-plex kits for a total of eight 384-plex panels.

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An overview of the Proximity extension assay, whereby matched pairs of antibodies with conjugated DNA oligos bind to a protein, hybridize, and create double stranded DNA which serves as a unique barcode for each protein. These barcodes are amplified and sequenced using NGS.

PEA uses matched pairs of antibodies (Abs) attached to unique DNA oligonucleotides for each protein. When the correct pair simultaneously bind to a target protein, their DNA oligonucleotides hybridize and serve as a unique template for DNA polymerase-dependent extension. This provides a double-stranded DNA molecule for each detected protein, providing a unique barcode for high-sensitivity, high-specificity data readout with NGS.

Olink Explore panels are available in both a 96-sample format as well as a 384-sample format. Compared to our Olink Target 96 products, Olink Explore offers a broader protein library with higher throughput at a lower cost.

  • 32-fold increase in the number of proteins measured simultaneously per run (from 96 to 3072)
  • 4-fold increase in the number of samples measured simultaneously per run (from 96 to 384)
  • a 128-fold increase in result throughput per run (using NovaSeq 6000)

How is the protein library designed?

Olink Explore is run in a 3072 assay format and includes an extensive set of control assays for quality control monitoring. The Explore library comprises a total of 2944 protein biomarkers, whereof 2926 are unique and 6 biomarkers are present in four panels for quality control reasons.

Three types of controls assay are utilized:

  1. 96 built-in “internal” control assays (12 in each 384-plex panel).
  2. Control samples are included in each run, adding a second layer of quality control.
  3. Finally, six biomarkers are available in quadruplicates and provides an additional control level. The assays IL-6, IL-8 (CXCL8) and TNF are included in the following panels: Cardiovascular, Inflammation, Oncology and Neurology. Similarly, the assays for IDO1, LMOD1 and SCRIB are included in the following panels: Cardiovascular II, Inflammation II, Oncology II and Neurology II.
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Protein categories in Olink Explore 3072

Our long-term goal is to cover the low-abundant dynamic plasma proteome in the best possible way. The strategy is to rapidly and intelligently drive library expansion by selecting biomarkers that contribute the most to key research questions and cover as many biological pathways and functions as possible. Five main categories of proteins were defined during target selection, and each assay can be classified into one or more of these categories:

  • Low-abundant inflammation proteins
  • Proteins actively secreted into blood circulation
  • Approved and ongoing drug target proteins
  • Organ-specific proteins that have leaked into blood circulation
  • Proteins representing more exploratory potential biomarkers.

Information from widely used open-access bioinformatic databases (including Uniprot, Human Protein Atlas, Gene Ontology (GO) and DisGeNET) and customer feedback was used as input in the target selection process. Olink’s strict validation requirements and quality standards complete this comprehensive inclusion criteria.

What is it for?

The Olink Explore platform enables our entire library of protein assays to be measured in eight 384-plex panels using approximately 1 µL of blood sample per 384-plex panel. This offering is flexible and scalable; customers can choose to screen the entire library, or to focus on one or several of the disease/biological process-focused 384-plex panels.

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Who should use it?

Scientists involved in drug development, clinical research or basic life science research who are looking to run large-scale discovery proteomics studies. Olink Explore is available as a fee-for-service offering to screen our entire library of nearly 3000 scalable and actionable protein biomarkers.

Samples can be run at any of these laboratories

2947

Biomarker assays

~475 million

Protein data points generated

662

Publications listed on website

35

Certified Core Labs

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