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2009-06-09
List of all Duolink Assays!
Olink Bioscience has created an Assay Database. Look for your target! Read more
successStories
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What can Duolink do for you?
Duolink®, based on PLA™, enables you to detect, localize and quantify proteins, protein interactions and phosphory-lations in fixed cells and tissue.
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New products
Duolink Detection Kit HRP/NovaRED now available.
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| Markus Bredel, MD, PhD, Northwestern University, Chicago | PLA is a standard in our lab representing a state of the art quantitative protein technology that has furthered our research on human brain tumors above and beyond classical protein techniques. | ![]() |
| Hans Matsson, PhD, Postdoc, Department of Biosciences and Nutrition, Karolinska Institutet, Sweden | In situ PLA has proven to be an important tool in our laboratory for the visualisation of novel protein-protein interactions in cell lines and primary cells from different species. | ![]() |
| Gethin Owen (PhD), Periodontal Biology Laboratory, Oral, Biol. Med Sci., Faculty of Dentistry, University of British Columbia, Vancouver, Canada | The availability of the Duolink system could not have come at a better time for my investigation of two novel proteins found to be present in cell focal adhesions. My research has established that both proteins co-localise using double immunofluroesecent labelling. However, there are many disadvantages to using immunofluorescent labelling for this particular application such as limits in the resolution of optical microscopy in addition to fluorescent bleed-through and cross-talk. As a result of these problems, complex analysis is required to statistically determine whether there is true co-localisation, and can be laborious and time consuming. Therefore, the in situ proximity ligation assay by Duolink is advantageous for this particular study in that it will confirm the results of the co-localisation study without the afore mentioned disadvantages. So far, I have found the system to be simple to use and have obtained positive results. | ![]() |
| David A. Cheresh, Ph.D., Professor and Vice Chair of Pathology, University of California, San Diego, Moores Cancer Center | The PLA kit allowed us to confirm in cells a receptor complex we had detected by immuno-precipitation analysis. This technique may ultimately prove to be equivalent to FRET but with the significant benefit of detecting endogenous protein-protein interactions. |




