Proseek® Multiplex enables faster, better-informed decisions in human protein biomarker research by providing high-multiplex immunoassays that do not compromise on either data quality or performance. This helps scientists to bring new insights into disease processes, improve disease detection, and contribute to a better understanding of biology.
Proseek Multiplex gives you:
Proseek Multiplex is able to achieve such a high level of multiplexing while maintaining exceptional data quality thanks to our proprietary Proximity Extension Assay (PEA) technology. Each biomarker is addressed by a matched pair of antibodies, coupled to unique, partially complementary oligonucleotides, and measured by quantitative real-time PCR. This dual recognition, DNA-coupled method provides exceptional readout specificity, since unlike in many other immunoassay formats, any antibody cross-reactivity that may occur during the multiplexed assay is excluded from the detection process.
Figure above: PEA technology enables high multiplexing levels without compromising on data quality
Proseek Multiplex can provide accurate quantification below picogram per milliliter levels even in small samples from, for example, needle biopsies or micro dialysis. This minimal sample consumption makes Proseek Multiplex especially suitable for retroactively mining precious biobanked serum samples for new multivariate biomarker signatures. The opportunities are enormous for powerful analysis of multivariate biomarker patterns. Proseek Multiplex reactions are quantified by real-time PCR using the Fluidigm BioMark™ HD real-time PCR platform. The assay generates as much as 9,216 datapoints per run which can then be analyzed with GeneEx software, equipped with the user-friendly Proseek Multiplex data import wizard.
Each Proseek Multiplex panel is focused on a specific area of disease or biology, targeting 92 established and exploratory biomarkers that have been carefully selected in collaboration with leading experts in the field. All assays are rigorously quality controlled and our validation data is made freely available. Currently there are four kits available on the market: Proseek Multiplex Oncology II 96x96, Proseek Multiplex CVD II 96x96, Proseek Multiplex CVD III 96x96,and Proseek Multiplex Inflammation I 96x96. A comprehensive list of all protein biomarkers that can be measured using the currently available Proseek Multiplex panels can be seen here: Go to Complete Biomarker List >>
If you prefer not to run the assays yourself, let our experts here at Olink do this for you via our fee-for-service offering.