Proseek Multiplex

Proseek® Multiplex is an innovative precision proteomics solution for targeted human protein biomarker discovery, providing high-multiplex immunoassays that do not compromise on either data quality or performance. This helps scientists to bring new insights into disease processes, improve disease detection, and contribute to a better understanding of biology. 

Linking our innovation to your success in human protein biomarker research

Proseek Multiplex gives you: 

  • High quality data you can trust - exceptional readout specificity from our Proximity Extension Assay (PEA) technology - effective QC tools built in to our assays
  • The high throughput your research deserves - 92 human protein biomarkers measured simultaneously with 96 samples in one panel - results in 24 hours 
  • The most effective use of your precious samples - only 1 µL sample consumed per panel - a wide range of biological sample types addressed 
  • The flexibility to best meet your needs - use our Proseek Multiplex kits in your own lab, or let our experts run the assays for you with the Proseek Multiplex Analysis Service 

Our innovative technology

Proseek Multiplex is able to achieve such a high level of multiplexing while maintaining exceptional data quality thanks to our proprietary Proximity Extension Assay (PEA) technology. Each biomarker is addressed by a matched pair of antibodies, coupled to unique, partially complementary oligonucleotides, and measured by quantitative real-time PCR. This dual recognition, DNA-coupled method provides exceptional readout specificity, since unlike in many other immunoassay formats, any antibody cross-reactivity that may occur during the multiplexed assay is excluded from the detection process.

Figure above: PEA technology enables high multiplexing levels without compromising on data quality

 Want to know more about how our innovative technology works? Go to our PEA technology page 

Proseek Multiplex capabilities

Proseek Multiplex can provide accurate quantification below picogram per milliliter levels even in small samples from, for example, needle biopsies or micro dialysis. This minimal sample consumption makes Proseek Multiplex especially suitable for retroactively mining precious biobanked serum samples for new multivariate biomarker signatures.  The opportunities are enormous for powerful analysis of multivariate biomarker patterns. Proseek Multiplex reactions are quantified by real-time PCR using the Fluidigm BioMark™ HD real-time PCR platform. The assay generates as much as 9,216 datapoints per run which can then be analyzed with GeneEx software, equipped with the user-friendly Proseek Multiplex data import wizard.

Proseek Multiplex panels

Each Proseek Multiplex panel is focused on a specific area of disease or biology, targeting 92 established and exploratory biomarkers that have been carefully selected in collaboration with leading experts in the field. All assays are rigorously quality controlled and our validation data is made freely available. Currently there are four kits available on the market: Proseek Multiplex Oncology II 96x96Proseek Multiplex CVD II 96x96, Proseek Multiplex CVD III 96x96,and Proseek Multiplex Inflammation I 96x96. A comprehensive list of all protein biomarkers that can be measured using the currently available Proseek Multiplex panels can be seen here: Go to Complete Biomarker List >>

If you prefer not to run the assays yourself, let our experts here at Olink do this for you via our fee-for-service offering.