Please note that the data analysis steps below will be taken care of for you if you decide to send your samples to our dedicated, highly skilled Analysis Service team, who will provide you with a comprehensive NPX data/quality control report after your study is completed.
If more extensive data analysis services are required, our team of Olink biostatisticians can help you with customized statistical analysis – see our statistical packages.
If you intend to run our panels in your own lab, or simply want to know more details, please read on below!
Detection and sample analysis is performed by high-throughput real-time PCR analysis using the Fluidigm® BioMark™ HD System. This PCR platform is a truly high capacity system which enables simultaneous detection of 96 analytes in 96 samples creating 9,216 datapoints from a single run. The BioMark HD platform uses microfluidic distribution of sample and assays requiring only 7 nL reactions and takes less than 3 hours to complete. The BioMark HD platform consists of a real-time PCR instrument, 96×96 Dynamic Array™ IFC for multiplexing the samples and biomarkers, and an IFC loader to load the 96×96 Dynamic Array IFC. More information can be found on the Fluidigm website.
(Fluidigm, BioMark, and Dynamic Array are trademarks or registered trademarks of Fluidigm Corporation).
Data generation and quality control with Olink® NPX Manager
For easier data analysis Olink Proteomics has developed a software, Olink NPX Manager, which can be used for data generated with the Olink panels and the Fluidigm BioMark real-time PCR instrument. This is an easy-to-use tool that lets you import, quality-control and normalize your Olink data, providing you with Normalized Protein eXpression (NPX) values for further data analysis.
If you want to purchase an Olink NPX Manager license, please contact your Business Development Manager at Olink Proteomics, or email firstname.lastname@example.org.
To obtain the biomarker list, import the panel-specific .plt file directly into the Fluidigm RT PCR software. Make sure to load the Primer Plate according to figure 3 in the User Manual. Please note that the detection setup.plt and setup.plt files should be saved to your computer first before imported into the Fluidigm RT PCR software. The .plt files can be downloaded below (right-click and “Save link as..” to your computer or device).
Please note that some of the panels have multiple .plt files available, corresponding to different versions of the panel. In these cases, the most recent .plt file is displayed first and the first 5 digits in the file name identify the product number of the relevant panel version.
For example, the file named 95600_84030_84031_CARDIOVASCULAR III.plt corresponds to the version of the panel Olink CARDIOVASCULAR III that had the product number 95600.