Towards a better understanding of cardiovascular disease and personalized medicine
The Cardiovascular III (CVD III) panel is a high-throughput, multiplex immunoassay enabling analysis of 92 CVD-related protein biomarkers across 96 samples simultaneously. This high level of multiplexing is achieved without any compromise on data quality, thanks to our proprietary Proximity Extension Assay (PEA) technology.
This panel contains known human cardiovascular and inflammatory markers as well as some exploratory human proteins with great potential as new CVD markers, which were carefully selected in collaboration with leading experts in the field. Each analyte in the panel has been assessed in terms of sample material, specificity, precision, sensitivity, dynamic range, matrix effects and interference. The assays on this panel have been selected to focus on high-abundance proteins, and 1 µL of a 1:100 dilution of sample is used to measure the 92 biomarkers.
Please Note: the CVD III panel uses a 1:100 dilution of sample, whereas our technical validation assays are performed in vitro using recombinant analytes . Technical validation data reported on the biomarker pages below, and in the data validation document for this panel are based on these in vitro assays. A multiplication factor of 100 should therefore be taken into consideration when comparing the addressable biological concentration to the in vitro validation data. (Download the sample dilution guideline document for this panel here)
An overview of how the 92 biomarker assays in the panel are classified according to Biological Process, Disease Area, Tissue Expression and Protein Class (based on widely used public-access bioinformatic databases, including Uniprot, Human Protein Atlas, Gene Ontology (GO) and DisGeNET) can be seen in the charts below. These charts are interactive: click on a chart-bar or pie-segment to see the list of biomarkers in that classification. Each item in the list links to the detailed biomarker page with validation data.
Note: Studies can be complemented with 92 additional established and exploratory CVD biomarkers using Proseek Multiplex CVD II. In this panel, assays are focused on low-abundance proteins.
Overview: Dynamic ranges of the two CVD panels.
A comprehensive list of all protein biomarkers that can be measured using the currently available Proseek Multiplex panels can be seen on the biomarker library page.
Note: A printable list of the biomarker assays available in this panel, as well as a full data validation document, can be downloaded from our Document download center >>
If you prefer not to run the assay yourself, let our experts here at Olink do this for you via our Proseek Multiplex Analysis Service.
The biomarker pages linked to below include calibrator curves that show the performance of each assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens, with data presented as Normalized Protein eXpression (NPX) values plotted against protein concentration (in pg/mL). Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please view the FAQ.