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Validation Data documents

Here you can access full validation data information for both current and discontinued Olink panels. Please note that the content of some panels may have been updated over time. The documents below refer to the most recently available version of the panel only. If you need a previous version of one of our validation data documents that refers to an older version of the panel, please contact us directly.

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Explore 3072

🔗 Olink Explore 3072 (xlsx) Updated December 1, 2021

Target 96 Cardiometabolic

Panel uses a 1:2025 dilution of sample. The measuring ranges shown in Figure 3 correspond to the concentrations in the original biological sample, whereas the figures quoted in Table 1 represent the actual concentration of recombinant analyte measurable in the validation assay. A multiplication factor of 2025 should therefore be taken into consideration when comparing the addressable biological concentration to the in vitro validation data.

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Target 96 CVD II

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Target 96 CVD III

Note: the CVD III panel uses a 1:100 dilution of sample. The measuring ranges shown in Figure 3 correspond to the concentrations in the original biological sample, whereas the figures quoted in Table 1 represent the actual concentration of recombinant analyte measurable in the validation assay. A multiplication factor of 100 should therefore be taken into consideration when comparing the addressable biological concentration to the in vitro validation data.

 

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Target 96 Cell Regulation

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Target 96 Development

Panel uses a 1:100 dilution of sample. The measuring ranges shown in Figure 3 correspond to the concentrations in the original biological sample, whereas the figures quoted in Table 1 represent the actual concentration of recombinant analyte measurable in the validation assay. A multiplication factor of 100 should therefore be taken into consideration when comparing the addressable biological concentration to the in vitro validation data.

 

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Target 96 Immune Response

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Target 96 Immuno-Oncology

Note that this version (2.1) applies to the latest panel version with article number 95311

 

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Target 96 Inflammation

Note that this version (3.0) applies to the latest panel version with article number 95302

 

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Target 96 Metabolism

Note: the Metabolism panel uses a 1:10 dilution of sample. The measuring ranges shown in Figure 3 correspond to the concentrations in the original biological sample, whereas the figures quoted in Table 1 represent the actual concentration of recombinant analyte measurable in the validation assay. A multiplication factor of 10 should therefore be taken into consideration when comparing the addressable biological concentration to the in vitro validation data.

 

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Target 96 Neuro Exploratory

Note that this version of the document (1.2) applies to the latest version of the panel with article number 95391 (released July 1, 2019)

 

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Target 96 Neurology

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Target 96 Oncology II

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Target 96 Oncology III

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Target 96 Organ Damage

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Target 96 Mouse Exploratory

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Target 48 Cytokine

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CVD I (discontinued)

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ONCOLOGY I, v2 (discontinued)

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Protein assay lists

Here you can access the complete biomarker assay list for each panel, listing the long name, short name, and UniProt number for each protein. For Olink Explore panels, the approved Gene name, recommended full Protein name, and the UniProt ID is presented. Since an individual protein may have a number of commonly used names or abbreviations, we recommend using the UniProt ID provided to search the UniProt website if there is any uncertainty.

To view all of the biomarker assays available in our current portfolio, please go to our Complete Biomarker List page, where you can also link directly to the individual biomarker pages that display the relevant validation data.

Please note that the content of some panels may have been updated over time. The documents below refer to the most recently available version of the panel only. If you need a previous version of one of our Biomarker list documents that refers to an older version of the panel, please contact us directly.

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Explore 3072 complete assay list

🔗 Assay List Olink Explore 3072 (Excel)

Explore 384 Cardiometabolic

Excel file

Explore 384 Inflammation

Excel file

Explore 384 Neurology

Excel file

Explore 384 Oncology

Excel file

Olink Explore assay names version history

Olink Explore assays are presented with the UniProt ID and approved Gene Names according to the official UniProt website.  At the beginning of  each year, the assay nomenclature is updated if needed according to the latest info from UniProt. All updates are presented in the version history document below:

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Target 96 Cardiometabolic

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Target 96 CVD II

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Target 96 Development

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Target 96 Immuno-Oncology

Note that this version (1.3) applies to the latest panel version with article number 95311

 

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Target 96 Metabolism

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Target 96 Neuro Exploratory

Note that this version of the document (1.1) applies to the latest version of the panel with article number 95391 (released July 1, 2019)

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Target 96 Oncology III

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CVD I (discontinued)

Reference list for customers who have previously used this discontinued panel

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Target 96 Cell Regulation

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Target 96 CVD III

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Target 96 Immune Response

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Target 96 Inflammation

Note that this version (1.3) applies to the latest panel version with article number 95302

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Target 96 Neurology

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Target 96 Oncology II

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Target 96 Organ Damage

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Target 96 Mouse Exploratory

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Target 48 Cytokine

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ONCOLOGY I v2 (discontinued)

Reference list for customers who have previously used this discontinued panel

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White Papers

Here you can find all the white papers that Olink have produced. These articles leverage the wealth of knowledge and experience in our research and development team, presenting you with useful technical and application-related information.

PEA – a high-multiplex immunoassay technology with qPCR or NGS readout

A short overview of how the unique technology behind Olink’s biomarker panels with NGS or qPCR readout works and how it overcomes the problems normally associated with multiplexed immunoassays.

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Appendix: Multiplex analysis of inflammatory proteins: A comparative study across multiple platforms

Additional comparative data from the study described in the original white paper from this study.

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Strategies for design of protein biomarker studies

This white paper describes important aspects of study design to consider when planning your future research.

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Pre-analytical variation in protein biomarker research

This paper provides some guidance on what to consider with respect to sample collection and handling when setting up a protein biomarker study.

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Multiplex analysis of inflammatory proteins: A comparative study across multiple platforms

This study compared the Olink® Target 48 Cytokine panel head-to-head with two commonly used commercial multiplex proteomics platforms, as well as to Olink Explore 1536

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Measuring protein biomarkers with Olink — technical comparisons and orthogonal validation

This white paper addresses how data from Olink studies compares to other technologies. We present examples of comparisons ranging from single-plex ELISAs to high multiplex assays and also provide useful tips for comparisons between Olink data and data from other technologies.

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Data normalization and standardization

This paper describes Olink’s built-in QC system, NPX calculation and definition, and the different normalization methods we use. Version 2.0, April 2021

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Development and validation of customized PEA biomarker panels with clinical utility

This describes how PEA can be applied to the development and validation of lower-plex assays that could help to translate protein signatures from discovery to clinical utility. The article shows a proof-of-concept study to design a custom panel including assays for both high and low abundance proteins, and providing absolute quantification.

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Application examples

Here you will find documents showing examples of data generated using specific Olink panels, or in specific application areas of interest.

Olink Mouse Exploratory - Pilot Studies

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Neurology Focus

Overview of the value of Olink’s panels in neurology research. Includes application examples.

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Technical notes

Here you can download our technical notes, which provide information on technical aspects of our panels

Extending Olink's Mouse Exploratory panel to studies in rats

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Olink® Explore readout on NovaSeq 6000 SP vs NextSeq 2000/550

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NovaSeq™ 6000 SP vs S4 flow cell comparison

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PEA specificity

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NovaSeq 6000 SP vs S1 flow cell comparison

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Brochure

Here you can download our brochure, which presents a concise overview of the Olink offering.

Olink brochure

🔗 Olink – One proteomics platform, endless possibilities (PDF)

Manuals & instructions

Below you will find all our technical user documentation, including manuals, short instructions and guidelines.

Olink Explore

Download the Olink® Explore Overview User Manual, as well as the user manuals applicable to the Olink Explore Reagent Kit and NGS instrument to be used.

🔗 Olink Explore Overview User Manual (PDF) Updated December 1, 2021

Olink Explore Reagent Kits

🔗 Olink Explore 384 User Manual (PDF) Updated December 1, 2021

🔗 Olink Explore 4 x 384 User Manual (PDF) Updated December 1, 2021

🔗 Olink Explore 1536 & Expansion User Manual (PDF) Updated December 1, 2021

🔗 Olink Explore 3072 User Manual (PDF) Updated December 1, 2021

NGS instruments

🔗 Olink Explore Sequencing using NextSeq 550 User Manual (PDF) Updated December 1, 2021

🔗 Olink Explore Sequencing using NextSeq 2000 User Manual (PDF) Updated December 1, 2021

🔗 Olink Explore Sequencing using NovaSeq 6000 User Manual (PDF) Updated December 1, 2021

Olink Target

🔗 Olink Target 48 User Manual (PDF)

🔗 Olink Target 96 User Manual (PDF) Updated Feb 7, 2019

🔗 Olink Target 96 Short Instructions (PDF)

Olink Signature Q100

🔗 Olink Signature Q100 – A benchtop system for protein biomarker analysis

🔗 Olink Signature Q100 Site Requirements

🔗 Olink Signature Q100 Installation Procedure

🔗 Olink Signature Q100 User Manual

Data Analysis NPX Manager Software

🔗 Data Analysis NPX Manager Software User Guide (PDF) Version 4.2.1, updated October 27, 2021

Olink NPX Signature Software

🔗 Olink NPX Signature User Manual

Olink Insights Stat Analysis


🔗 Olink Insights Stat Analysis User Guide (PDF)

Olink MyData Cloud Software User Guide

🔗 Olink MyData Cloud Software User Guide (PDF) Version 1.2, Updated May 10, 2021

Safety Data Sheets (SDS)

Please view our safety information documents relating to the different components of our products.

SDS Olink Explore

🔗 SDS Olink Explore plate control (PDF) v. 1.0

🔗 SDS Olink Explore Sample prep 1.0 (PDF) v. 1.0

🔗 SDS Olink Explore Sample prep 1.1 (PDF) v. 1.0

🔗 SDS Olink Explore Sample prep 1.2 (PDF) v. 1.0

🔗 SDS Olink Explore Sample prep 1.3 (PDF) v. 1.0

🔗 SDS Olink Explore Probe kit and Sample Prep 2 (PDF) v. 1.0

SDS Olink Target

🔗 SDS Olink Target 48 panels (PDF)

🔗 SDS Olink Target 96 probe kit (PDF)

🔗 SDS Olink Target 96 control reagents (PDF)

🔗 SDS Olink Target 96 detection reagents (PDF)

SDS IFCs for Protein Expression

🔗 SDS IFCs for Protein Expression – Control Line Fluid (PDF)

🔗 SDS IFCs for Protein Expression – Pressure Fluid (PDF)

🔗 SDS IFCs for Protein Expression – Actuation Fluid (PDF)

2947

Biomarker assays

~475 million

Protein data points generated

662

Publications listed on website

35

Certified Core Labs

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