The most comprehensive, high-performance protein biomarker panel available for inflammation studies
Please note that the information here refers to the latest version of this panel (article number 95302), released on March 30, 2019 – see more here.
Inflammation is a biological process of central importance for biomedical research and is now believed to be a key underlying factor for the pathophysiology of a wide range of diseases, from “classical” inflammatory conditions (e.g. IBD and dermatological diseases) to cardiovascular disease and cancer. Our Inflammation panel provides a high-throughput, multiplex immunoassay enabling analysis of 92 inflammation-related protein biomarkers across 96 samples simultaneously. This high level of multiplexing is achieved without any compromise on data quality, thanks to our proprietary Proximity Extension Assay (PEA) technology.
This represents the most extensive panel available on the market for proteins associated with inflammatory diseases and related biological processes, enabling investigation of protein signatures with high efficiency and robustness and accelerates the speed of finding new and relevant human protein biomarkers related to inflammation. The panel is compiled to detect a selection of already established as well as exploratory biomarkers within the inflammation research field. The content of the panel has been designed in close collaboration with experts within various disease areas such as rheumatoid arthritis, Crohn’s disease, ulcerative colitis, neuro inflammation, and respiratory diseases, and it is well suited to discerning protein profiles in clinically relevant samples.
New April 2019: After listening to feedback from our customers, we have made important improvements to two of the key assays in the inflammation panel
- Tumor Necrosis Factor (TNF) Uniprot number P01375. The previous assay for this analyte, (also known as TNFα) was specific for the biologically active trimeric version of the protein only, and exhibited relatively low detectabilty in many samples. Since the majority of commercially available assays also detect TNF monomers and our customers have expressed a strong wish to be able to measure all forms of the protein that may be present in serum/plasma, we have developed a new assay that does just this. In our validation experiments, the new assay exhibits significantly higher detectability in samples from both patients and healthy controls
- Interferon Gamma (IFN-gamma) Uniprot number P01579. Based on customer feedback that a higher level of detectability would be appreciated for this important inflammatory marker, we have worked hard to optimize and improve this assay to ensure a significantly higher level of detectability (in samples from both patients and healthy controls)
The detectabilty and dynamic ranges of both these assays have been improved without any compromise on specificity or any other performance characteristics
Olink INFLAMMATION is our most popular and most highly cited panel in the literature to date, and we are delighted to be able to improve it still further with the help of constructive feedback from our highly engaged users. To see a direct comparison of the performance improvements for the two assays described above, please visit the Inflammation panel upgrade information page.
An overview of how the 92 biomarker assays in the panel are classified according to Biological Process, Disease Area, Tissue Expression and Protein Class (based on widely used public-access bioinformatic databases, including Uniprot, Human Protein Atlas, Gene Ontology (GO) and DisGeNET) can be seen in the charts below. These charts are interactive: click on a chart-bar or pie-segment to see the list of biomarkers in that classification. Each item in the list links to the detailed biomarker page with validation data.
For optimal assay performance, each immunoassay in the panel has been assessed in terms of specificity, precision, sensitivity, dynamic range, matrix effects and interference in biological material.
A comprehensive list of all protein biomarkers that can be measured using the currently available Proseek Multiplex panels can be seen on the biomarker library page.
Note: A printable list of the biomarker assays available in this panel, as well as a full data validation document, can be downloaded from our Document download center >>
If you prefer not to run the assay yourself, let our experts here at Olink do this for you via our Analysis Service.
The biomarker pages linked to below include calibrator curves that show the performance of each assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens, with data presented as Normalized Protein eXpression (NPX) values plotted against protein concentration (in pg/mL). Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please view the FAQ.