Tumor necrosis factor (TNF)
Links to databases
TNF (TNF-alpha) is a cytokine that upon binding to its receptors TNFRSF1A and TNFRSF1B activates NF-kappaB, MAPK, but also in a lower level apoptosis signaling pathways. TNF plays important roles in systemic inflammation and acute phase reaction and it has been described to be involved in several inflammation-related diseases such as fever, psoriatic arthritis, inflammatory bowel disease, rheumatoid arthritis, etc. Note: This assay does not detect the low levels of TNFa present in plasma from healthy individuals. The assay does, however, detect TNF in serum and plasma samples from several inflammatory diseases where higher levels of this proteins are expressed. One example is the study by Brunner et al. where TNF is measured both in patients with atopic dermatitis and psoriasis.
Brunner et al. The atopic dermatitis blood signature is characterized by increases in inflammatory and cardiovascular risk proteins. Sci Rep. 2017; 7: 8707.
Recommended sample types are EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact firstname.lastname@example.org for more information.
Analytical Measuring Range
Please note: the technical data reported below refers to the measured value in the in vitro validation assays. The calibrator curve below shows the performance of the assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please visit our FAQ page (https://www.olink.com/question/what-is-npx).
Calibrator curve generated in multiplex
- Within run precision Coefficient of Variation (CV)
- Between run precision Coefficient of Variation (CV)
Precision (repeatability) is calculated from linearized NPX values over LOD.
Biomarker Validation Data
Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents. To download or to learn more go to the Data Validation page.