Olink Explore 1536 / 384
Measure 1,536 proteins with minimal sample volume. Unparalleled specificity & sensitivity of PEA technology. Readout on NGS.
The Promise of Proteomics
Watch the film (3:19)
What is Olink Explore?
Olink® Explore 1536/384 is a high-multiplex, high-throughput protein biomarker platform that uses Proximity Extension Assay (PEA) technology coupled to an innovative new readout methodology based on Next Generation Sequencing (NGS) using the following Illumina® instruments:
- NovaSeq 6000
- NextSeq 550
- NextSeq 2000
PEA uses matched pairs of antibodies (Abs) attached to unique DNA oligonucleotides. When the correct pair simultaneously binds to a target protein, their DNA oligonucleotides hybridize and serve as a unique template for DNA polymerase-dependent extension. This provides a double-stranded DNA molecule for each detected protein, providing a unique barcode for high-sensitivity, high-specificity readout. The well-established Olink Target 96-panels use qPCR for the readout step, but marrying our elegant PEA technology with NGS for readout in Olink Explore provides several advantages:
- 4-fold increase in multiplexing (from 96-plex to 384-plex)
- 16-fold increase in the number of assays per run (from 96 to 1536)
- a 6.5-fold increase in throughput
To see more about PEA technology with NGS and qPCR readout and the quality controls (QC) built in to the assays, please read our white paper.
View a new animated video explaining the principles of PEA with NGS and qPCR readout.
Accelerating proteomics together
Olink® Explore 1536 marks a pivotal moment for proteomics. Technical challenges have long troubled the field, trustworthy specificity, and sensitivity, robust results, accurate precision, and of course, issues around scalability and throughput. Those challenges are now a thing of the past.- High throughput1.35 million protein measurements/week/system
- High multiplex1536 proteins measured in 384-plex assays
- Exceptional specificityDual recognition & DNA barcoding from PEA
- Minimal sample consumptionJust 2.8 µL for the complete library
- Broad dynamic rangeAssays span 10 logs (fg/ml - µg/mL)
PEA on NGS
Using the Next Generation Sequencing (NGS) platform from Illumina Inc., we can run 1.3 million protein measurements per system per week. This level of throughput has simply been unachievable before. Also provides a higher multiplex level, and lower cost per protein data point.
How was the protein library designed?
Olink Explore 1536 includes assays for 1472 proteins and 48 controls, built into four 384-plex panels. This comprises 1463 unique proteins, as the assays for IL-6, IL-8 (CXCL8) and TNF are included for quality control purposes in each of the four 384-plex panels that make up the complete library.
Protein categories in Olink Explore 1536
Our long-term goal is to cover the low-abundant dynamic plasma proteome in the best possible way. The strategy is to rapidly and intelligently drive library expansion by selecting biomarkers that contribute the most to key research questions and cover as many biological pathways and functions as possible. Five main categories of proteins were defined during target selection, and each assay can be classified into one or more of these:
- Low-abundant inflammation proteins
- Proteins actively secreted into the circulation
- Approved and ongoing drug target proteins
- Organ-specific proteins that have leaked into the circulation
- Proteins representing more exploratory potential biomarkers
Information from widely used open-access bioinformatic databases (including Uniprot, Human Protein Atlas, Gene Ontology (GO) and DisGeNET) and customer feedback was used as input in the target selection process. Olink’s strict validation requirements and quality standards constitute the final inclusion criteria.
What is it for?
The Olink Explore platform enables our entire library of protein assays to be measured in a 4 x 384-plex format using less than 3 µL of blood sample to identify actionable protein profiles that give meaningful insights into real-time human biology and facilitate the development of more effective, targeted therapies. Reflecting the flexibility and scalability of our offering, customers can choose to screen the entire library, or to focus on one or more of the four disease/biological process-focused 384-plex panels.
More details on the 384-plex panels can be found via the links below:
Olink® Explore 384 Cardiometabolic
Olink® Explore 384 Inflammation
Who should use it?
Scientists involved in drug development, clinical research or basic life science research who are looking to run large-scale discovery studies to address the low abundant plasma proteome. Olink Explore is available as a fee-for-service offering to screen our entire library of more than 1460 proteins, for studies using plasma, serum or CSF samples only. Samples can be run by our Analysis Service labs in Boston, MA or Uppsala, Sweden. Olink Explore kits can also now be purchased directly for running in labs with the necessary equipment and training – for more details, please contact us.
For an overview of the practical details involved in ordering a study, sending samples and receiving results using Olink Explore 1536, PLEASE READ OUR GUIDE.
For those looking to run more targeted biomarker studies and/or sample matrices other than plasma, serum or CSF, we recommend our portfolio of well-established Olink Target panels (96-plex with qPCR readout).
Want to know more?
Additional technical information about Olink Explore is available on this website – READ OUR FAQs
Contact us
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