Olink Explore

Measure up to 3072 proteins with minimal sample volume. Unparalleled specificity & sensitivity of PEA technology. Readout on NGS.

DUOX2
VSTM2L
HEBP1
NPPB
HK2
MTPN
FGFR1O
CEBPB
PPIE
ACTA2
TNNI3
CRX
EDIL3
CHEK2
AKR1C4
HNRNPK
RCOR1
CA5A
TIA1
ZBTB17
CRHR1
VAMP5
TSPAN1
TSLP
SUSD1
FABP6
VIM
SERPINB5
GRK5
TNF
CNPY2
CTF1
MFAP3
TCL1B
STK11
PTN
DUOX2
VSTM2L
HEBP1
NPPB
HK2
MTPN
FGFR1O
CEBPB
PPIE
ACTA2
TNNI3
CRX
EDIL3
CHEK2
AKR1C4
HNRNPK
RCOR1
CA5A
TIA1
ZBTB17
CRHR1
VAMP5
TSPAN1
TSLP
SUSD1
FABP6
VIM
SERPINB5
GRK5
TNF
CNPY2
CTF1
MFAP3
TCL1B
STK11
PTN
TIA1
PPIE
ZBTB17
CA5A
TSLP
EDIL3
ACTA2
TNF
FABP6
VSTM2L
STK11
TSPAN1
VIM
NPPB
HEBP1
SUSD1
MTPN
HNRNPK
TNNI3
TCL1B
HK2
SERPINB5
CRX
CHEK2
VAMP5
RCOR1
CEBPB
PTN
GRK5
CNPY2
AKR1C4
MFAP3
CTF1
CRHR1
FGFR1O
DUOX2
TIA1
PPIE
ZBTB17
CA5A
TSLP
EDIL3
ACTA2
TNF
FABP6
VSTM2L
STK11
TSPAN1
VIM
NPPB
HEBP1
SUSD1
MTPN
HNRNPK
TNNI3
TCL1B
HK2
SERPINB5
CRX
CHEK2
VAMP5
RCOR1
CEBPB
PTN
GRK5
CNPY2
AKR1C4
MFAP3
CTF1
CRHR1
FGFR1O
DUOX2
CNPY2
TCL1B
FABP6
MFAP3
ZBTB17
TSPAN1
VSTM2L
TNF
PTN
SERPINB5
SUSD1
HNRNPK
FGFR1O
VAMP5
CHEK2
HK2
CA5A
PPIE
CEBPB
TIA1
MTPN
STK11
CRHR1
DUOX2
CRX
TSLP
ACTA2
NPPB
CTF1
HEBP1
TNNI3
RCOR1
GRK5
EDIL3
VIM
AKR1C4
CNPY2
TCL1B
FABP6
MFAP3
ZBTB17
TSPAN1
VSTM2L
TNF
PTN
SERPINB5
SUSD1
HNRNPK
FGFR1O
VAMP5
CHEK2
HK2
CA5A
PPIE
CEBPB
TIA1
MTPN
STK11
CRHR1
DUOX2
CRX
TSLP
ACTA2
NPPB
CTF1
HEBP1
TNNI3
RCOR1
GRK5
EDIL3
VIM
AKR1C4
CHEK2
TNNI3
TCL1B
ZBTB17
CEBPB
TSLP
TSPAN1
STK11
CNPY2
RCOR1
PPIE
PTN
CRX
SUSD1
NPPB
EDIL3
GRK5
CTF1
HNRNPK
FABP6
CRHR1
VIM
ACTA2
FGFR1O
VAMP5
DUOX2
HK2
HEBP1
MFAP3
VSTM2L
CA5A
AKR1C4
SERPINB5
TNF
MTPN
TIA1
CHEK2
TNNI3
TCL1B
ZBTB17
CEBPB
TSLP
TSPAN1
STK11
CNPY2
RCOR1
PPIE
PTN
CRX
SUSD1
NPPB
EDIL3
GRK5
CTF1
HNRNPK
FABP6
CRHR1
VIM
ACTA2
FGFR1O
VAMP5
DUOX2
HK2
HEBP1
MFAP3
VSTM2L
CA5A
AKR1C4
SERPINB5
TNF
MTPN
TIA1
TCL1B
HEBP1
CA5A
TIA1
FABP6
SUSD1
VIM
HK2
PTN
EDIL3
TSLP
RCOR1
FGFR1O
CNPY2
MTPN
HNRNPK
VAMP5
VSTM2L
GRK5
STK11
ZBTB17
CRHR1
SERPINB5
CEBPB
MFAP3
NPPB
TNNI3
AKR1C4
CHEK2
TSPAN1
DUOX2
PPIE
CRX
TNF
CTF1
ACTA2
TCL1B
HEBP1
CA5A
TIA1
FABP6
SUSD1
VIM
HK2
PTN
EDIL3
TSLP
RCOR1
FGFR1O
CNPY2
MTPN
HNRNPK
VAMP5
VSTM2L
GRK5
STK11
ZBTB17
CRHR1
SERPINB5
CEBPB
MFAP3
NPPB
TNNI3
AKR1C4
CHEK2
TSPAN1
DUOX2
PPIE
CRX
TNF
CTF1
ACTA2
CRHR1
TSLP
CRX
TIA1
TSPAN1
CTF1
TCL1B
VSTM2L
ZBTB17
PPIE
PTN
VIM
CHEK2
CNPY2
CA5A
DUOX2
RCOR1
MTPN
SERPINB5
FABP6
SUSD1
MFAP3
HEBP1
EDIL3
HK2
NPPB
GRK5
FGFR1O
ACTA2
VAMP5
TNNI3
CEBPB
AKR1C4
TNF
STK11
HNRNPK
CRHR1
TSLP
CRX
TIA1
TSPAN1
CTF1
TCL1B
VSTM2L
ZBTB17
PPIE
PTN
VIM
CHEK2
CNPY2
CA5A
DUOX2
RCOR1
MTPN
SERPINB5
FABP6
SUSD1
MFAP3
HEBP1
EDIL3
HK2
NPPB
GRK5
FGFR1O
ACTA2
VAMP5
TNNI3
CEBPB
AKR1C4
TNF
STK11
HNRNPK
MFAP3
RCOR1
PPIE
CTF1
TCL1B
ZBTB17
TIA1
STK11
TNF
CEBPB
HEBP1
HNRNPK
NPPB
CHEK2
EDIL3
PTN
CRHR1
MTPN
AKR1C4
CRX
CA5A
TSLP
VAMP5
HK2
ACTA2
DUOX2
SERPINB5
GRK5
SUSD1
VSTM2L
TNNI3
FABP6
CNPY2
TSPAN1
VIM
FGFR1O
MFAP3
RCOR1
PPIE
CTF1
TCL1B
ZBTB17
TIA1
STK11
TNF
CEBPB
HEBP1
HNRNPK
NPPB
CHEK2
EDIL3
PTN
CRHR1
MTPN
AKR1C4
CRX
CA5A
TSLP
VAMP5
HK2
ACTA2
DUOX2
SERPINB5
GRK5
SUSD1
VSTM2L
TNNI3
FABP6
CNPY2
TSPAN1
VIM
FGFR1O
VIM
TNNI3
VSTM2L
SERPINB5
RCOR1
STK11
EDIL3
CRX
PTN
TCL1B
ACTA2
NPPB
TNF
SUSD1
ZBTB17
MTPN
HEBP1
CNPY2
TSPAN1
CA5A
PPIE
TSLP
CEBPB
MFAP3
GRK5
TIA1
HNRNPK
DUOX2
HK2
FGFR1O
CTF1
VAMP5
FABP6
CRHR1
AKR1C4
CHEK2
VIM
TNNI3
VSTM2L
SERPINB5
RCOR1
STK11
EDIL3
CRX
PTN
TCL1B
ACTA2
NPPB
TNF
SUSD1
ZBTB17
MTPN
HEBP1
CNPY2
TSPAN1
CA5A
PPIE
TSLP
CEBPB
MFAP3
GRK5
TIA1
HNRNPK
DUOX2
HK2
FGFR1O
CTF1
VAMP5
FABP6
CRHR1
AKR1C4
CHEK2
ZBTB17
EDIL3
STK11
HNRNPK
VAMP5
CNPY2
RCOR1
CEBPB
CA5A
TSLP
VSTM2L
AKR1C4
GRK5
MTPN
TSPAN1
CRX
DUOX2
CTF1
CRHR1
ACTA2
TNNI3
HK2
SERPINB5
HEBP1
TNF
FABP6
TIA1
PPIE
NPPB
PTN
CHEK2
VIM
FGFR1O
TCL1B
MFAP3
SUSD1
ZBTB17
EDIL3
STK11
HNRNPK
VAMP5
CNPY2
RCOR1
CEBPB
CA5A
TSLP
VSTM2L
AKR1C4
GRK5
MTPN
TSPAN1
CRX
DUOX2
CTF1
CRHR1
ACTA2
TNNI3
HK2
SERPINB5
HEBP1
TNF
FABP6
TIA1
PPIE
NPPB
PTN
CHEK2
VIM
FGFR1O
TCL1B
MFAP3
SUSD1
TSPAN1
HK2
PPIE
PTN
CHEK2
SUSD1
DUOX2
NPPB
EDIL3
STK11
CRX
CNPY2
CRHR1
AKR1C4
ACTA2
TNF
SERPINB5
CTF1
VIM
ZBTB17
TCL1B
TSLP
FABP6
VAMP5
CEBPB
HNRNPK
MTPN
MFAP3
TNNI3
CA5A
RCOR1
HEBP1
VSTM2L
FGFR1O
GRK5
TIA1
TSPAN1
HK2
PPIE
PTN
CHEK2
SUSD1
DUOX2
NPPB
EDIL3
STK11
CRX
CNPY2
CRHR1
AKR1C4
ACTA2
TNF
SERPINB5
CTF1
VIM
ZBTB17
TCL1B
TSLP
FABP6
VAMP5
CEBPB
HNRNPK
MTPN
MFAP3
TNNI3
CA5A
RCOR1
HEBP1
VSTM2L
FGFR1O
GRK5
TIA1

The Promise of Proteomics

Watch the film (3:19)

What is Olink Explore?

Olink® Explore 1536/384 is a high-multiplex, high-throughput protein biomarker platform that uses Proximity Extension Assay (PEA) technology coupled to an innovative new readout methodology based on Next Generation Sequencing (NGS) using the following Illumina® instruments:

  • NovaSeq 6000
  • NextSeq 550
  • NextSeq 2000

PEA uses matched pairs of antibodies (Abs) attached to unique DNA oligonucleotides. When the correct pair simultaneously binds to a target protein, their DNA oligonucleotides hybridize and serve as a unique template for DNA polymerase-dependent extension. This provides a double-stranded DNA molecule for each detected protein, providing a unique barcode for high-sensitivity, high-specificity readout with NGS. Compared to our Target 96 products, Olink Explore offers a broader protein library and higher throughput with a lower cost

  • 4-fold increase in the number of proteins measured simultaneously (from 96-plex to 384-plex)
  • 4-fold increase in the number of samples measured simultaneously (from 96 to 384 per run)
  • a 6.5-fold increase in throughput

To see more about PEA technology with NGS and qPCR readout and the quality controls (QC) built in to the assays, please read our white paper.

View a new animated video explaining the principles of PEA with NGS and qPCR readout.

Accelerating proteomics together

Olink® Explore 1536 marks a pivotal moment for proteomics. Technical challenges have long troubled the field, trustworthy specificity, and sensitivity, robust results, accurate precision, and of course, issues around scalability and throughput. Those challenges are now a thing of the past.
  • High throughput1.35 million protein measurements/week/system
  • High multiplex1536 proteins measured in 384-plex assays
  • Exceptional specificityDual recognition & DNA barcoding from PEA
  • Minimal sample consumptionJust 2.8 µL for the complete library
  • Broad dynamic rangeAssays span 10 logs (fg/ml - µg/mL)

PEA on NGS

Using the Next Generation Sequencing (NGS) platform from Illumina Inc., we can run 1.3 million protein measurements per system per week. This level of throughput has simply been unachievable before. Also provides a higher multiplex level, and lower cost per protein data point.

How was the protein library designed?

Olink Explore currently includes assays for 1472 proteins and 48 controls, built into four 384-plex panels. This comprises 1463 unique proteins, as the assays for IL-6, IL-8 (CXCL8) and TNF are included for quality control purposes in each of the four 384-plex panels that make up the complete library.

Protein categories in Olink Explore 1536

Our long-term goal is to cover the low-abundant dynamic plasma proteome in the best possible way. The strategy is to rapidly and intelligently drive library expansion by selecting biomarkers that contribute the most to key research questions and cover as many biological pathways and functions as possible. Five main categories of proteins were defined during target selection, and each assay can be classified into one or more of these:

  • Low-abundant inflammation proteins
  • Proteins actively secreted into the circulation
  • Approved and ongoing drug target proteins
  • Organ-specific proteins that have leaked into the circulation
  • Proteins representing more exploratory potential biomarkers

Information from widely used open-access bioinformatic databases (including UniprotHuman Protein AtlasGene Ontology (GO) and DisGeNET) and customer feedback was used as input in the target selection process. Olink’s strict validation requirements and quality standards constitute the final inclusion criteria.

What is it for?

The Olink Explore platform enables our entire library of  protein assays to be measured in a four 384-plex panels using about 1 µL of blood sample per 384-plex panel. Reflecting the flexibility and scalability of our offering, customers can choose to screen the entire library, or to focus on one or more of the four disease/biological process-focused 384-plex panels.

More details on the 384-plex panels can be found via the links below:

Olink® Explore 384 Cardiometabolic

Olink® Explore 384 Inflammation

Olink® Explore 384 Neurology

Olink® Explore 384 Oncology

Who should use it?

Scientists involved in drug development, clinical research or basic life science research who are looking to run large-scale discovery studies to address the low abundant plasma proteome. Olink Explore is available as a fee-for-service offering to screen our entire library of more than 1460 proteins. Samples can be run by our Analysis Service labs in Boston, MA or Uppsala, Sweden. Olink Explore kits can also now be purchased directly for running in labs with the necessary equipment and training – for more details, please contact us.

For an overview of the practical details involved in ordering a study, sending samples and receiving results using Olink Explore, PLEASE READ OUR GUIDE.

For those looking to run more targeted biomarker studies and/or sample matrices other than plasma, serum or CSF, we recommend our portfolio of well-established Olink Target panels (96-plex with qPCR readout).

Want to know more?

Additional technical information about Olink Explore is available on this website – READ OUR FAQs

Contact us

If you have additional questions or comments about the Olink Explore platform, please use the form below: