How was the protein library designed?
Olink Explore 1536 includes assays for 1472 proteins and 48 controls, built into four 384-plex panels. This comprises 1463 unique proteins, as the assays for IL-6, IL-8 (CXCL8) and TNF are included for quality control purposes in each of the four 384-plex panels that make up the complete library.
Protein categories in Olink Explore 1536
Our long-term goal is to cover the low-abundant dynamic plasma proteome in the best possible way. The strategy is to rapidly and intelligently drive library expansion by selecting biomarkers that contribute the most to key research questions and cover as many biological pathways and functions as possible. Five main categories of proteins were defined during target selection, and each assay can be classified into one or more of these:
- Low-abundant inflammation proteins
- Proteins actively secreted into the circulation
- Approved and ongoing drug target proteins
- Organ-specific proteins that have leaked into the circulation
- Proteins representing more exploratory potential biomarkers
Information from widely used open-access bioinformatic databases (including Uniprot, Human Protein Atlas, Gene Ontology (GO) and DisGeNET) and customer feedback was used as input in the target selection process. Olink’s strict validation requirements and quality standards constitute the final inclusion criteria.
What is it for?
The Olink Explore platform enables our entire library of protein assays to be measured in a 4 x 384-plex format using less than 3 µL of blood sample to identify actionable protein profiles that give meaningful insights into real-time human biology and facilitate the development of more effective, targeted therapies. Reflecting the flexibility and scalability of our offering, customers can choose to screen the entire library, or to focus on one or more of the four disease/biological process-focused 384-plex panels.
More details on the 384-plex panels can be found via the links below:
Olink® Explore 384 Cardiometabolic
Olink® Explore 384 Inflammation
Olink® Explore 384 Neurology
Olink® Explore 384 Oncology
Who should use it?
Scientists involved in drug development, clinical research or basic life science research who are looking to run large-scale discovery studies to address the low abundant plasma proteome. Olink Explore is available as a fee-for-service offering to screen our entire library of more than 1460 proteins, for studies using plasma, serum or CSF samples only. Samples can be run by our Analysis Service labs in Boston, MA or Uppsala, Sweden. Olink Explore kits can also now be purchased directly for running in labs with the necessary equipment and training – for more details, please contact us.
For an overview of the practical details involved in ordering a study, sending samples and receiving results using Olink Explore 1536, PLEASE READ OUR GUIDE.
For those looking to run more targeted biomarker studies and/or sample matrices other than plasma, serum or CSF, we recommend our portfolio of well-established Olink Target panels (96-plex with qPCR readout).
Want to know more?
Additional technical information about Olink Explore is available on this website – READ OUR FAQs
If you have additional questions or comments about the Olink Explore platform, please use the link below: